Kimura Zen-ichiro, Okabe Satoshi
Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, Hokkaida, Japan.
J Gen Appl Microbiol. 2013;59(4):261-6. doi: 10.2323/jgam.59.261.
A Gram-negative, non-spore-forming, rod-shaped bacterial strain, AR20(T), was isolated from anodic biofilms of an acetate-fed microbial fuel cell in Japan and subjected to a polyphasic taxonomic study. Strain AR20(T) grew optimally at pH 7.0-8.0 and 25°C. It contained Q-8 as the predominant ubiquinone and C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:02OH), and C18:1ω7c as the major fatty acids. The DNA G+C content was 67.1 mol%. A neighbor-joining phylogenetic tree revealed that strain AR20(T) clustered with three type strains of the genus Hydrogenophaga (H. flava, H. bisanensis and H. pseudoflava). Strain AR20(T) exhibited 16S rRNA gene sequence similarity values of 95.8-97.7% to the type strains of the genus Hydrogenophaga. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain AR20(T) is considered a novel species of the genus Hydrogenophaga, for which the name Hydrogenophaga electricum sp. nov. is proposed. The type strain is AR20(T) (= KCTC 32195(T) = NBRC 109341(T)).
从日本一个以乙酸盐为食的微生物燃料电池的阳极生物膜中分离出一株革兰氏阴性、无芽孢、杆状细菌菌株AR20(T)，并对其进行了多相分类研究。菌株AR20(T)在pH 7.0 - 8.0和25°C条件下生长最佳。它以Q - 8作为主要泛醌，并以C16:0、总和特征3（C16:1ω7c和/或异C15:0 2-OH）以及C18:1ω7c作为主要脂肪酸。DNA G + C含量为67.1 mol%。邻接法系统发育树显示，菌株AR20(T)与嗜氢菌属的三株模式菌株（淡黄嗜氢菌、比山嗜氢菌和假淡黄嗜氢菌）聚类在一起。菌株AR20(T)与嗜氢菌属模式菌株的16S rRNA基因序列相似性值为95.8 - 97.7%。基于表型、化学分类和系统发育数据，菌株AR20(T)被认为是嗜氢菌属的一个新物种，为此提出名为嗜氢电杆菌（Hydrogenophaga electricum）的新种。模式菌株为AR20(T)（= KCTC 32195(T) = NBRC 109341(T)）。
