Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3716-3721. doi: 10.1099/ijsem.0.002146. Epub 2017 Sep 13.
A polyphasic approach was used to characterize an aerobic, Gram-negative, rod-shaped bacterium (designated strain CC-KL-3) isolated from a hot spring. Phylogenetic analyses based on 16S rRNA genes indicated that strain CC-KL-3 showed highest sequence similarity to Hydrogenophaga bisanensis (97.7 %) and Hydrogenophaga atypica (97.6 %) and lower sequence similarity to other species (less than 97.6 %). The levels of DNA-DNA relatedness between strain CC-KL-3, H. bisanensis and H. atypica were estimated to be 13.0 and 8.7 % (the reciprocal value was 14.7 and 6.3 %). Strain CC-KL-3 was non-motile, without apparent flagella and able to grow between 15-42 °C (optimal 30 °С), pH 6.0-8.0 (optimal 7.0) and 0-2 % (w/v) NaCl (optimal 0 %). The DNA G+C content was 61.4 mol% and the major quinone system was ubiquinone (Q-8). The polyamine profile revealed the predominance of 2-hydroxyputrescine and putrescine and the dominant cellular fatty acids were C16 : 0 (28.9 %), C16 : 1ω7c/C16 : 1ω6c (41.4 %) and C18 : 1ω7c/C18 : 1ω6c (11.9 %). These data corroborated the affiliation of strain CC-KL-3 to the genus Hydrogenophaga. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits, and the results of comparative 16S rRNA gene sequence analysis, strain CC-KL-3 is considered to represent a novel species of the genus Hydrogenophaga, affiliated to the family Comamonadaceae, for which the name Hydrogenophaga aquatica sp. nov. is proposed. The type strain is CC-KL-3 (=BCRC 80937=JCM 31216).
采用多相分类法对一株好氧、革兰氏阴性、杆状细菌（命名为 CC-KL-3 菌株）进行了研究，该细菌分离自温泉。基于 16S rRNA 基因的系统发育分析表明，CC-KL-3 菌株与 Hydrogenophaga bisanensis（97.7%）和 Hydrogenophaga atypica（97.6%）的序列相似度最高，与其他物种（低于 97.6%）的序列相似度较低。CC-KL-3 菌株、H. bisanensis 和 H. atypica 之间的 DNA-DNA 同源性水平估计为 13.0%和 8.7%（倒数分别为 14.7%和 6.3%）。CC-KL-3 菌株为非运动性、无明显鞭毛，可在 15-42°C（最佳 30°C）、pH6.0-8.0（最佳 7.0）和 0-2%（w/v）NaCl（最佳 0%）下生长。DNA G+C 含量为 61.4mol%，主要醌类为 ubiquinone（Q-8）。多胺图谱显示 2-羟基腐胺和腐胺占优势，主要细胞脂肪酸为 C16:0（28.9%）、C16:1ω7c/C16:1ω6c（41.4%）和 C18:1ω7c/C18:1ω6c（11.9%）。这些数据证实了 CC-KL-3 菌株与 Hydrogenophaga 属的亲缘关系。基于独特的系统发育、表型和化学分类特征以及比较 16S rRNA 基因序列分析的结果，CC-KL-3 菌株被认为代表了 Hydrogenophaga 属的一个新种，属于 Comamonadaceae 科，建议将其命名为 Hydrogenophaga aquatica sp. nov.。模式菌株为 CC-KL-3（=BCRC 80937=JCM 31216）。
