一种用于检测蛋白质-代谢物相互作用以鉴定天然核受体配体的代谢组学策略。
A metabolomics strategy for detecting protein-metabolite interactions to identify natural nuclear receptor ligands.
作者信息
Kim Yun-Gon, Lou Angela C, Saghatelian Alan
机构信息
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA, USA.
出版信息
Mol Biosyst. 2011 Apr;7(4):1046-9. doi: 10.1039/c0mb00324g. Epub 2011 Jan 31.
PMID:21283866
Abstract
Nuclear receptors are ligand-regulated transcription factors that control a range of (patho)physiological processes. Lipids are the most common type of nuclear receptor ligands. Here, we utilize a metabolomics approach that detects protein-metabolite interactions to identify endogenous peroxisome proliferator-activated receptor (PPAR) ligands from tissue and cell extracts.
摘要
核受体是受配体调节的转录因子，可控制一系列（病理）生理过程。脂质是最常见的核受体配体类型。在此，我们采用一种代谢组学方法来检测蛋白质-代谢物相互作用，以从组织和细胞提取物中鉴定内源性过氧化物酶体增殖物激活受体（PPAR）配体。
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