Department of Zoology, D. D. U. Gorakhpur University, Gorakhpur, Uttar Pradesh, India.
Eur Rev Med Pharmacol Sci. 2010 Nov;14(11):915-23.
The leaf and bark of Thevetia peruviana (Family: Apocynaceae) plant was administered for 24 h to the freshwater fish Catla catla (Hamilton) to evaluate their piscicidal activity in laboratory and cemented pond condition.
The LC0 values of lef and bark extracts of different solvents (i.e., acetone, diethyl ether, ethyl alcohol, chloroform and carbon tetrachloride) of this plant to fish Catla catla were determined. The LC50 values of acetone leaf extract of Thevetia peruviana plant is 88.80 mg/L (24h) in laboratory condition and 529.38 mg/L (24h) in cemented pond condition; acetone bark extract of this plant is 99.43 mg/L (24h) in laboratory condition and 591.78 mg/L (24h) in cemented pond condition against freshwater fish Catla catla. Similar trend was also observed in case of other solvent (i.e., diethyl ether, ethyl alcohol, chloroform and carbon tetrachloride) of leaf and bark extracts of Thevetia peruviana plant against freshwater fish Catla catla in laboratory and cemented pond conditions. The acetone leaf and bark extract of this plant was very effective in comparison to other solvent extract in both the conditions. So, the biochemical analysis is taken only acetone leaf and bark extract of Thevetia peruviana plant in laboratory condition.
Exposure of sub-lethal doses (40% and 80% of LC,) of acetone leaf and bark extract of this plant over 24 h caused significant (P < 0.05) alterations in total protein, free amino acids, DNA & RNA, protease and acid and alkaline phosphatase activity in muscle, liver and gonadal tissues of fish Catla catla in laboratory condition.
将桐叶藤（夹竹桃科）的叶和皮在 24 小时内施用于淡水鱼 Catla catla（Hamilton），以评估其在实验室和水泥池塘条件下的杀鱼活性。
测定了该植物不同溶剂（丙酮、二乙醚、乙醇、氯仿和四氯化碳）的叶和皮提取物对 Catla catla 鱼的 LC0 值。在实验室条件下，桐叶藤丙酮叶提取物的 LC50 值为 88.80mg/L（24h），在水泥池塘条件下为 529.38mg/L（24h）；该植物丙酮树皮提取物在实验室条件下为 99.43mg/L（24h），在水泥池塘条件下为 591.78mg/L（24h），均对淡水鱼 Catla catla 有效。在实验室和水泥池塘条件下，桐叶藤叶和皮的其他溶剂（二乙醚、乙醇、氯仿和四氯化碳）提取物对淡水鱼 Catla catla 也表现出类似的趋势。与其他溶剂提取物相比，该植物的丙酮叶和树皮提取物在两种条件下都非常有效。因此，仅在实验室条件下对桐叶藤丙酮叶和树皮提取物进行了生化分析。
在 24 小时内暴露于亚致死剂量（LC 的 40%和 80%）的丙酮叶和树皮提取物会导致淡水鱼 Catla catla 的肌肉、肝脏和性腺组织中的总蛋白、游离氨基酸、DNA 和 RNA、蛋白酶和酸碱性磷酸酶活性发生显著变化（P <0.05）在实验室条件下。
