Department of Medical Sciences/Section of Clinical Bacteriology, Uppsala University, Sweden.
APMIS. 2011 Mar;119(3):198-203. doi: 10.1111/j.1600-0463.2010.02710.x.
In this study, three swab transport systems were evaluated: M40 Transystem, Amies broth with a relatively new type of swab (both Copan Diagnostics, Corona, CA, USA), and SSI transportmedium (Statens Serum Institut, Copenhagen Denmark). The CLSI M40-A standard procedures and 11 culture collection strains were used. The transport systems were tested at room temperature for holding times of 0, 24, and 48 h, and both mono- and polymicrobial samples were included. After 24 h of simulated transportation, all systems were able to maintain the viability of all organisms tested. SSI transportmedium exhibited the lowest maintaining ability, whereas the two Copan systems were the most growth-promoting system. In polymicrobial samples, this latter feature was a problem. At 48 h, no transport system could maintain the viability of all strains, and the recovery rates differed depending on organism and device. The species most difficult to recover in all the three systems was Neisseria gonorrhoeae. When selecting a swab transport system, consideration must be given to the sample type, the conditions that prevail locally, and the performance in the clinical setting.
在这项研究中，评估了三种拭子运输系统：M40 Transystem、带有新型拭子的 Amies 肉汤（均来自美国加利福尼亚州科罗纳市的 Copan Diagnostics）和 SSI 运输培养基（丹麦哥本哈根的 Statens Serum Institut）。使用了 CLSI M40-A 标准程序和 11 株培养物收集株。在室温下测试了这些运输系统，保持时间分别为 0、24 和 48 小时，包括单种和混合微生物样本。在模拟运输 24 小时后，所有系统都能够维持所有测试生物的存活能力。SSI 运输培养基显示出最低的维持能力，而两个 Copan 系统是最具促进生长能力的系统。在混合微生物样本中，后一个特点是一个问题。在 48 小时时，没有任何运输系统能够维持所有菌株的存活能力，并且回收率取决于生物体和设备而有所不同。在所有三个系统中最难恢复的物种是淋病奈瑟菌。在选择拭子运输系统时，必须考虑样本类型、当地流行的条件以及临床环境中的性能。
