人类分娩中下降阻滞的分子特征。
A molecular signature of an arrest of descent in human parturition.
机构信息
Perinatology Research Branch, National Institute of Child Health and Human Development/National Institutes of Health/Department of Health and Human Services, Bethesda, MD, USA.
出版信息
Am J Obstet Gynecol. 2011 Feb;204(2):177.e15-33. doi: 10.1016/j.ajog.2010.09.025.
Abstract
OBJECTIVE
This study was undertaken to identify the molecular basis of an arrest of descent.
STUDY DESIGN
Human myometrium was obtained from women in term labor (TL; n = 29) and arrest of descent (AODes; n = 21). Gene expression was characterized using Illumina HumanHT-12 microarrays. A moderated Student t test and false discovery rate adjustment were applied for analysis. Confirmatory quantitative reverse transcription-polymerase chain reaction and immunoblot were performed in an independent sample set.
RESULTS
Four hundred genes were differentially expressed between women with an AODes compared with those with TL. Gene Ontology analysis indicated enrichment of biological processes and molecular functions related to inflammation and muscle function. Impacted pathways included inflammation and the actin cytoskeleton. Overexpression of hypoxia inducible factor-1a, interleukin -6, and prostaglandin-endoperoxide synthase 2 in AODes was confirmed.
CONCLUSION
We have identified a stereotypic pattern of gene expression in the myometrium of women with an arrest of descent. This represents the first study examining the molecular basis of an arrest of descent using a genome-wide approach.
摘要
目的
本研究旨在确定下降阻滞的分子基础。
研究设计
从足月分娩（TL；n=29）和下降阻滞（AODes；n=21）的女性中获取人子宫肌组织。使用 Illumina HumanHT-12 微阵列对基因表达进行特征分析。采用经过修正的学生 t 检验和错误发现率调整进行分析。在独立样本集中进行了定量逆转录聚合酶链反应和免疫印迹验证。
结果
与 TL 妇女相比，AODes 妇女的 400 个基因表达存在差异。基因本体论分析表明，与炎症和肌肉功能相关的生物学过程和分子功能富集。受影响的途径包括炎症和肌动蛋白细胞骨架。在 AODes 中证实了缺氧诱导因子-1a、白细胞介素-6 和前列腺素内过氧化物合酶 2 的过表达。
结论
我们已经确定了下降阻滞女性子宫肌中基因表达的典型模式。这是首次使用全基因组方法研究下降阻滞的分子基础。
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