State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Peking Union Medical College/Chinese Academy of Medical Sciences, No. 9 Dong Dan San Tiao, Dongcheng District, Beijing 100730, People's Republic of China.
Eur J Clin Microbiol Infect Dis. 2011 Jun;30(6):799-805. doi: 10.1007/s10096-011-1159-4. Epub 2011 Feb 1.
Since its first identification in 2005, four species of human bocavirus (HBoV1-4) have been documented. HBoV1 and HBoV2 have been shown to be associated with respiratory tract illnesses, as well as with acute gastroenteritis (AGE), worldwide. However, reports on the prevalence, clinical significance, and molecular characteristics of the two most newly identified HBoV species, HBoV3 and HBoV4, are very limited. To detect and characterize HBoV3 and HBoV4 infections in children with AGE in China, stool specimens were collected from 366 children with AGE. HBoVs in these samples were amplified by nested polymerase chain reaction (PCR), sequenced, and phylogenetically analyzed. HBoVs were detected in 44 samples (12%), of which nine were HBoV1, 33 were HBoV2, and two were HBoV3. HBoV4 was not detected. Most HBoV-positive samples (35/44) were co-detected with other viral pathogens. Both HBoV3 samples were co-detected with rotavirus. Analysis of the HBoV3 (46-BJ07) genome sequence indicates that HBoV3 may be a recombinant derived from HBoV1 and HBoV2 or from HBoV1 and HBoV4. To our knowledge, this is the first report of HBoV3 in China. However, it is unclear whether HBoV3 is associated with AGE because of its low detection rate in AGE patients and its co-infection with other AGE-causing viruses.
自 2005 年首次鉴定以来，已发现四种人类博卡病毒（HBoV1-4）。HBoV1 和 HBoV2 已被证明与呼吸道疾病以及全球急性胃肠炎（AGE）有关。然而，关于两种最新鉴定的 HBoV 物种 HBoV3 和 HBoV4 的流行率、临床意义和分子特征的报告非常有限。为了在中国 AGE 患儿中检测和鉴定 HBoV3 和 HBoV4 感染，收集了 366 例 AGE 患儿的粪便标本。通过巢式聚合酶链反应（PCR）扩增这些样本中的 HBoV，测序，并进行系统进化分析。在 44 份样本（12%）中检测到 HBoVs，其中 9 份为 HBoV1，33 份为 HBoV2，2 份为 HBoV3。未检测到 HBoV4。大多数 HBoV 阳性样本（35/44）与其他病毒病原体共同检测到。两个 HBoV3 样本均与轮状病毒共同检测到。对 HBoV3（46-BJ07）基因组序列的分析表明，HBoV3 可能是源自 HBoV1 和 HBoV2 或源自 HBoV1 和 HBoV4 的重组病毒。据我们所知，这是中国首次报告 HBoV3。然而，由于 HBoV3 在 AGE 患者中的低检出率及其与其他引起 AGE 的病毒的共同感染，尚不清楚 HBoV3 是否与 AGE 有关。
