人博卡病毒 2 的系统发生和重组分析。
Phylogenetic and recombination analysis of human bocavirus 2.
机构信息
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, PR China.
出版信息
BMC Infect Dis. 2011 Feb 24;11:50. doi: 10.1186/1471-2334-11-50.
BACKGROUND
Human bocavirus 2(HBoV2) and other human bocavirus species (HBoV, HBoV3, and HBoV4) have been discovered recently. But the precise phylogenetic relationships among these viruses are not clear yet.
METHODS
We collected 632 diarrhea and 162 healthy children in Lanzhou, China. Using PCR, Human bocavirus (HBoV), HBoV2, HBoV3 and HBoV4 were screened. The partial genes of NS, NP1 and VP, and two nearly complete sequences of HBoV2 were obtained.
RESULT
Phylogenetic analysis showed the different genes of HBoV2 strain were homogenous with different reference strains. HBoV3 may be a recombinant derived from HBoV and HBoV4. We also observed that the VP1 and VP2 region of HBoV3 is as similar to HBoV2 as to HBoV4.
CONCLUSIONS
A single genetic lineage of HBoV2 is circulating in children with and without gastroenteritis in Lanzhou, China. Current evidence in this study was not enough to support recombination between HBoV2 strains, and HBoV3 may be a recombinant between HBoV and the common ancestor of HBoV2 and HBoV4.
背景
人类博卡病毒 2 型（HBoV2）和其他人类博卡病毒种（HBoV、HBoV3 和 HBoV4）最近被发现。但这些病毒之间的确切系统进化关系尚不清楚。
方法
我们在中国兰州收集了 632 例腹泻和 162 例健康儿童的粪便样本。采用 PCR 法，筛选人博卡病毒（HBoV）、HBoV2、HBoV3 和 HBoV4。获得了 NS、NP1 和 VP 的部分基因和两个近乎完整的 HBoV2 序列。
结果
系统进化分析显示，HBoV2 株的不同基因与不同参考株具有同源性。HBoV3 可能是 HBoV 和 HBoV4 的重组体。我们还观察到 HBoV3 的 VP1 和 VP2 区与 HBoV2 相似，而与 HBoV4 相似。
结论
在中国兰州，胃肠炎患儿和健康儿童中均存在单一遗传谱系的 HBoV2。本研究目前的证据不足以支持 HBoV2 株之间的重组，HBoV3 可能是 HBoV 和 HBoV2 与 HBoV4 的共同祖先之间的重组体。
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