2012-2018 年泰国清迈儿科急性胃肠炎患者中流行的人博卡病毒基因型 1、2 和 3。

Human bocavirus genotypes 1, 2, and 3 circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand, 2012-2018.

机构信息

Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.

Faculty of Agricultural Technology, Chiang Mai Rajabhat University, Chiang Mai, Thailand.

出版信息

J Infect Public Health. 2021 Feb;14(2):179-186. doi: 10.1016/j.jiph.2020.12.007. Epub 2021 Jan 22.

DOI:10.1016/j.jiph.2020.12.007

PMID:33486373

Abstract

BACKGROUND

Global distribution of human bocavirus (HBoV) has been known to associate with viral gastroenteritis in pediatric population. This study was conducted in Chiang Mai, Thailand from 2012 to 2018 to investigate epidemiology and genotype distribution of HBoV in pediatric patients less than 5 years old hospitalized with diarrhea.

METHODS

A total of 2727 fecal specimens were investigated for the presence of HBoV using nested-PCR targeting partial VP1 capsid region. The detected HBoV strains were further characterized by nucleotide sequencing and phylogenetic analysis.

RESULTS

Detection rate of HBoV infection in pediatric patients with acute diarrhea was 5.2%. Three genotypes of HBoV were detected with the most predominance of HBoV1 (50.4%), followed by HBoV2 (42.5%), and HBoV3 (7.1%). The majority of HBoV positive cases were children of 1 to <2 years old (31.9%) with high detection rate of HBoV1 and HBoV2. HBoV infection occurred all year-round. Phylogenetic analysis revealed that majority of HBoV1 displayed the genetic relationship with HBoV1 strains reported previously from Asia whereas only a few were related to the strains from Europe, South America, and Middle East. The HBoV2 and HBoV3 were also mainly closely related to the strains reported from Asia and a few from South America and North Africa.

CONCLUSIONS

This study highlights distribution of HBoV genotypes circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand. Overall, three genotypes of HBoV were detected with equally high prevalence of HBoV1 and HBoV2 whereas HBoV3 was detected with much lower prevalence.

摘要

背景

人类博卡病毒（HBoV）的全球分布与儿科人群中的病毒性胃肠炎有关。本研究于 2012 年至 2018 年在泰国清迈进行，旨在调查小于 5 岁因腹泻住院的儿科患者中 HBoV 的流行病学和基因型分布。

方法

使用针对 VP1 衣壳区部分的巢式-PCR 检测 2727 份粪便标本中 HBoV 的存在。进一步对检测到的 HBoV 株进行核苷酸测序和系统进化分析。

结果

急性腹泻儿科患者中 HBoV 感染的检出率为 5.2%。共检测到 3 种 HBoV 基因型，其中以 HBoV1 最为常见（50.4%），其次是 HBoV2（42.5%）和 HBoV3（7.1%）。大多数 HBoV 阳性病例为 1 至<2 岁的儿童（31.9%），HBoV1 和 HBoV2 的检出率较高。HBoV 感染全年均可发生。系统进化分析显示，大多数 HBoV1 与亚洲先前报道的 HBoV1 株具有遗传关系，而少数与欧洲、南美洲和中东的株有关。HBoV2 和 HBoV3 也主要与亚洲报道的株密切相关，少数与南美洲和北非的株有关。