Dasgupta J, Rao R S, Kanungo R
Department of Microbiology, JIPMER, Pondicherry.
Indian J Pathol Microbiol. 1990 Jul;33(3):239-43.
A total of 123 cases of acute pyogenic meningitis were studied to investigate whether counter current immunoelectrophoresis (CIEP) would facilitate rapid etiological diagnosis when used with other routine methods in a clinical bacteriology laboratory. Of the 123 cases, 53 (43.08 percent) were culture positive. Streptococcus pneumoniae was the commonest etiological agent accounting for 30 (56.6 percent) of the isolates. There were four isolates of Haemophilus influenzae and one of Neisseria meningitidis. High rates of isolation were found from turbid CSF samples, those showing a high polymorphonuclear cellular reaction and from those with a high protein and a low sugar level. CIEP detected antigen in 50 percent of cases of Haemophilus influenzae and 76.7 percent of cases of Streptococcus pneumoniae.
共研究了123例急性化脓性脑膜炎病例，以调查在临床细菌学实验室中，当对流免疫电泳（CIEP）与其他常规方法一起使用时，是否有助于快速进行病因诊断。在这123例病例中，53例（43.08%）培养呈阳性。肺炎链球菌是最常见的病原体，占分离株的30例（56.6%）。有4株流感嗜血杆菌和1株脑膜炎奈瑟菌分离株。在浑浊的脑脊液样本、显示多形核细胞反应高的样本以及蛋白质水平高和糖水平低的样本中发现了较高的分离率。CIEP在50%的流感嗜血杆菌病例和76.7%的肺炎链球菌病例中检测到了抗原。
