Collison Lauren W, Vignali Dario A A
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Methods Mol Biol. 2011;707:21-37. doi: 10.1007/978-1-61737-979-6_2.
Determining the activity of a regulatory T-cell population in vitro is often the first step in analyzing its function. To obtain reliable and reproducible results, it is critical to follow the protocol that is most applicable to your experimental question. We have outlined below a basic in vitro suppression assay as well as a variety of alternative/additional protocols that can be utilized alone or in combination as desired.
在体外确定调节性T细胞群体的活性通常是分析其功能的第一步。为了获得可靠且可重复的结果，遵循最适用于您实验问题的方案至关重要。我们在下面概述了一种基本的体外抑制试验以及多种可单独或根据需要组合使用的替代/附加方案。
