带血管蒂的卵巢整体冷冻保存：玻璃化法还是传统冷冻法？
Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?
机构信息
Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, China.
出版信息
J Assist Reprod Genet. 2011 May;28(5):445-52. doi: 10.1007/s10815-011-9539-3. Epub 2011 Feb 3.
Abstract
PURPOSE
To compare the efficacy of vitrification and conventional freezing of whole ovaries.
METHODS
Ovaries obtained from 5-year-old female bovines were cryopreserved by conventional freezing, rapid freezing and vitrification. The ovarian cortical strips were cryopreserved by conventional freezing. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles, hormones concentrations in the culture supernatants, and lactate dehyrogenase levels were measured.
RESULTS
The efficacy of cryopreservation of whole ovaries by vitrification was higher than those by conventional freezing and rapid freezing. Conventional freezing of ovarian cortical strips was more effective than cryopreservation of whole ovaries by conventional freezing, rapid freezing, and vitrification.
CONCLUSIONS
Vitrification seems to be more suitable than conventional freezing for cryopreservation of whole ovaries. However, further studies are required to improve the efficacy of vitrifying whole ovaries.
摘要
目的
比较玻璃化和常规冷冻保存整个卵巢的效果。
方法
对 5 岁雌性牛的卵巢进行常规冷冻、快速冷冻和玻璃化冷冻保存。对卵巢皮质条带进行常规冷冻保存。采用台盼蓝试验评估卵泡活力；测量形态正常原始卵泡的百分比、培养上清液中的激素浓度和乳酸脱氢酶水平。
结果
玻璃化冷冻保存整个卵巢的效果优于常规冷冻和快速冷冻。卵巢皮质条带的常规冷冻保存效果优于常规冷冻、快速冷冻和玻璃化冷冻保存整个卵巢的效果。
结论
玻璃化似乎比常规冷冻更适合于整个卵巢的冷冻保存。然而，需要进一步的研究来提高整个卵巢玻璃化的效果。
相似文献
1
Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?
J Assist Reprod Genet. 2011 May;28(5):445-52. doi: 10.1007/s10815-011-9539-3. Epub 2011 Feb 3.
2
Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification.
Fertil Steril. 2012 May;97(5):1176-82. doi: 10.1016/j.fertnstert.2012.01.123. Epub 2012 Feb 16.
3
Follicular viability and histological assessment after cryopreservation of whole sheep ovaries with vascular pedicle by vitrification.
Fertil Steril. 2005 Oct;84 Suppl 2:1065-71. doi: 10.1016/j.fertnstert.2005.03.079.
4
Safeguarding Fertility With Whole Ovary Cryopreservation and Microvascular Transplantation: Higher Follicular Survival With Vitrification Than With Slow Freezing in a Ewe Model.
Transplantation. 2016 Sep;100(9):1889-97. doi: 10.1097/TP.0000000000001296.
5
Assessment of ovarian tissue and follicular integrity after cryopreservation via slow freezing or vitrification followed by in vitro culture.
F S Sci. 2024 May;5(2):154-162. doi: 10.1016/j.xfss.2023.10.004. Epub 2023 Oct 29.
6
Cryobanking of human ovarian tissue for anti-cancer treatment: comparison of vitrification and conventional freezing.
Cryo Letters. 2009 Nov-Dec;30(6):449-54.
7
Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival.
Hum Reprod. 2014 Apr;29(4):720-30. doi: 10.1093/humrep/det449. Epub 2013 Dec 22.
8
In vitro survival of follicles in prepubertal ewe ovarian cortex cryopreserved by slow freezing or non-equilibrium vitrification.
J Assist Reprod Genet. 2019 Sep;36(9):1823-1835. doi: 10.1007/s10815-019-01532-8. Epub 2019 Aug 2.
9
Beneficial effect of directional freezing on in vitro viability of cryopreserved sheep whole ovaries and ovarian cortical slices.
Hum Reprod. 2014 Jan;29(1):114-24. doi: 10.1093/humrep/det377. Epub 2013 Oct 17.
10
Comparison of open and a novel closed vitrification system with slow freezing for human ovarian tissue cryopreservation.
J Assist Reprod Genet. 2021 Oct;38(10):2723-2733. doi: 10.1007/s10815-021-02297-9. Epub 2021 Aug 16.
引用本文的文献
1
De novo design of a nanoregulator for the dynamic restoration of ovarian tissue in cryopreservation and transplantation.
J Nanobiotechnology. 2024 Jun 11;22(1):330. doi: 10.1186/s12951-024-02602-5.
2
Genomic Consideration in Chemotherapy-Induced Ovarian Damage and Fertility Preservation.
Genes (Basel). 2021 Sep 28;12(10):1525. doi: 10.3390/genes12101525.
3
Molecular Mechanism and Prevention Strategy of Chemotherapy- and Radiotherapy-Induced Ovarian Damage.
Int J Mol Sci. 2021 Jul 13;22(14):7484. doi: 10.3390/ijms22147484.
4
Current and Future Perspectives for Improving Ovarian Tissue Cryopreservation and Transplantation Outcomes for Cancer Patients.
Reprod Sci. 2021 Jun;28(6):1746-1758. doi: 10.1007/s43032-021-00517-2. Epub 2021 Mar 31.
5
Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans.
J Clin Med. 2020 Oct 2;9(10):3196. doi: 10.3390/jcm9103196.
6
State of the Art in Fertility Preservation for Female Patients Prior to Oncologic Therapies.
Medicina (Kaunas). 2020 Feb 23;56(2):89. doi: 10.3390/medicina56020089.
7
Fertility preservation through gonadal cryopreservation.
Reprod Med Biol. 2016 Mar 11;15(4):235-251. doi: 10.1007/s12522-016-0240-1. eCollection 2016 Oct.
8
New advances in ovarian autotransplantation to restore fertility in cancer patients.
Cancer Metastasis Rev. 2015 Dec;34(4):807-822. doi: 10.1007/s10555-015-9600-2.
9
Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant.
J Assist Reprod Genet. 2015 Aug;32(8):1267-75. doi: 10.1007/s10815-015-0513-3. Epub 2015 Jun 19.
10
Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model.
J Assist Reprod Genet. 2012 Nov;29(11):1255-60. doi: 10.1007/s10815-012-9845-4. Epub 2012 Aug 17.
本文引用的文献
1
Time to re-think: ovarian tissue transplantation versus whole ovary transplantation.
Reprod Biomed Online. 2010 Feb;20(2):171-4. doi: 10.1016/j.rbmo.2009.11.019. Epub 2009 Nov 29.
2
Ovarian tissue thawing: a comparison of two conditions.
Fertil Steril. 2010 Jan;93(1):307-10. doi: 10.1016/j.fertnstert.2009.07.967. Epub 2009 Aug 14.
3
Ovarian endocrine profile and long-term vascular patency following heterotopic autotransplantation of cryopreserved whole ovine ovaries.
Hum Reprod. 2009 Nov;24(11):2845-55. doi: 10.1093/humrep/dep274. Epub 2009 Jul 29.
4
Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation.
Reproduction. 2009 Aug;138(2):319-27. doi: 10.1530/REP-09-0039. Epub 2009 May 13.
5
Human ovarian tissue: vitrification versus conventional freezing.
Hum Reprod. 2009 Jul;24(7):1767-8; author reply 1768-9. doi: 10.1093/humrep/dep094. Epub 2009 Apr 22.
6
Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue.
Hum Reprod. 2009 Jul;24(7):1670-83. doi: 10.1093/humrep/dep079. Epub 2009 Apr 9.
7
Difficulties improving ovarian functional recovery by microvascular transplantation and whole ovary vitrification.
Fertil Steril. 2009 Jun;91(6):2697-706. doi: 10.1016/j.fertnstert.2008.03.012. Epub 2008 Apr 28.
8
Optimal perfusion of an intact ovary as a prerequisite for successful ovarian cryopreservation.
Hum Reprod. 2008 Feb;23(2):329-35. doi: 10.1093/humrep/dem384. Epub 2007 Dec 2.
9
Effects of different freezing parameters on the morphology and viability of preantral follicles after cryopreservation of doe rabbit ovarian tissue.
Fertil Steril. 2008 May;89(5 Suppl):1348-56. doi: 10.1016/j.fertnstert.2007.03.046. Epub 2007 Jun 29.
10
Effect of choline-supplemented sodium-depleted slow freezing versus vitrification on mouse oocyte meiotic spindles and chromosome abnormalities.
Fertil Steril. 2007 Oct;88(4 Suppl):1093-100. doi: 10.1016/j.fertnstert.2006.12.066. Epub 2007 Jun 4.
Zotero 插件