带血管蒂的卵巢整体冷冻保存：玻璃化法还是传统冷冻法？
Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?
机构信息
Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, China.
出版信息
J Assist Reprod Genet. 2011 May;28(5):445-52. doi: 10.1007/s10815-011-9539-3. Epub 2011 Feb 3.
Abstract
PURPOSE
To compare the efficacy of vitrification and conventional freezing of whole ovaries.
METHODS
Ovaries obtained from 5-year-old female bovines were cryopreserved by conventional freezing, rapid freezing and vitrification. The ovarian cortical strips were cryopreserved by conventional freezing. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles, hormones concentrations in the culture supernatants, and lactate dehyrogenase levels were measured.
RESULTS
The efficacy of cryopreservation of whole ovaries by vitrification was higher than those by conventional freezing and rapid freezing. Conventional freezing of ovarian cortical strips was more effective than cryopreservation of whole ovaries by conventional freezing, rapid freezing, and vitrification.
CONCLUSIONS
Vitrification seems to be more suitable than conventional freezing for cryopreservation of whole ovaries. However, further studies are required to improve the efficacy of vitrifying whole ovaries.
摘要
目的
比较玻璃化和常规冷冻保存整个卵巢的效果。
方法
对 5 岁雌性牛的卵巢进行常规冷冻、快速冷冻和玻璃化冷冻保存。对卵巢皮质条带进行常规冷冻保存。采用台盼蓝试验评估卵泡活力；测量形态正常原始卵泡的百分比、培养上清液中的激素浓度和乳酸脱氢酶水平。
结果
玻璃化冷冻保存整个卵巢的效果优于常规冷冻和快速冷冻。卵巢皮质条带的常规冷冻保存效果优于常规冷冻、快速冷冻和玻璃化冷冻保存整个卵巢的效果。
结论
玻璃化似乎比常规冷冻更适合于整个卵巢的冷冻保存。然而，需要进一步的研究来提高整个卵巢玻璃化的效果。
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