优化完整卵巢的冷冻保护剂灌注条件：牛模型。
Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model.
机构信息
Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, China, 250000.
出版信息
J Assist Reprod Genet. 2012 Nov;29(11):1255-60. doi: 10.1007/s10815-012-9845-4. Epub 2012 Aug 17.
Abstract
PURPOSE
The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation
METHODS
Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured.
RESULTS
When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols.
CONCLUSION
Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.
摘要
目的
本研究旨在探讨不同灌流压和不同灌流时间对整个卵巢冷冻保存的影响。
方法
对牛整个卵巢进行玻璃化-解冻。根据不同的灌流压力和不同的灌流时间将卵巢分为实验组。通过台盼蓝试验评估卵泡活力；测量培养上清液中形态正常原始卵泡的百分比和 17-β 雌二醇水平。
结果
当灌流压力为 100mmHg，灌流时间为 40min 时，牛整个卵巢冷冻保存的卵巢组织活力高于其他方案。
结论
方案 IIb（灌流压力为 100mmHg，灌流时间为 40min）适用于牛整个卵巢的冷冻保存。
相似文献
1
Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model.优化完整卵巢的冷冻保护剂灌注条件：牛模型。
J Assist Reprod Genet. 2012 Nov;29(11):1255-60. doi: 10.1007/s10815-012-9845-4. Epub 2012 Aug 17.
2
Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival.小鼠卵巢组织冷冻保存的最佳玻璃化方案：冷冻保护剂和体外培养对玻璃化复温后卵巢组织存活的影响。
Hum Reprod. 2014 Apr;29(4):720-30. doi: 10.1093/humrep/det449. Epub 2013 Dec 22.
3
Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries.冷却速率和冰核温度对整个卵巢冷冻保存的影响。
J Assist Reprod Genet. 2011 Jul;28(7):627-33. doi: 10.1007/s10815-011-9557-1. Epub 2011 Mar 23.
4
Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification.冻存大鼠卵巢的活力和功能：慢速冻与玻璃化的比较。
Fertil Steril. 2012 May;97(5):1176-82. doi: 10.1016/j.fertnstert.2012.01.123. Epub 2012 Feb 16.
5
Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?带血管蒂的卵巢整体冷冻保存：玻璃化法还是传统冷冻法？
J Assist Reprod Genet. 2011 May;28(5):445-52. doi: 10.1007/s10815-011-9539-3. Epub 2011 Feb 3.
6
[Ovarian tissue vitrification: Cortex and whole ovary in sheep].[绵羊卵巢组织玻璃化冷冻：皮质与整个卵巢]
Gynecol Obstet Fertil. 2006 Sep;34(9):746-53. doi: 10.1016/j.gyobfe.2006.07.024. Epub 2006 Sep 8.
7
Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols.暴露于冷冻保护剂并采用不同冷冻方案进行冷冻保存后绵羊卵巢的卵泡活力和形态
Fertil Steril. 2001 Apr;75(4):754-62. doi: 10.1016/s0015-0282(00)01787-8.
8
Dimethyl sulfoxide perfusion in caprine ovarian tissue and its relationship with follicular viability after cryopreservation.二甲基亚砜对山羊卵巢组织的灌注及其与冷冻保存后卵泡活力的关系。
Fertil Steril. 2009 Apr;91(4 Suppl):1513-5. doi: 10.1016/j.fertnstert.2008.07.1778. Epub 2008 Oct 18.
9
10
Follicular viability and histological assessment after cryopreservation of whole sheep ovaries with vascular pedicle by vitrification.玻璃化冷冻保存带血管蒂的整只绵羊卵巢后的卵泡活力及组织学评估
Fertil Steril. 2005 Oct;84 Suppl 2:1065-71. doi: 10.1016/j.fertnstert.2005.03.079.
引用本文的文献
1
Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans.全卵巢冷冻保存与移植：动物实验和人类研究中挑战与研究进展的系统综述
J Clin Med. 2020 Oct 2;9(10):3196. doi: 10.3390/jcm9103196.
2
Dimethyl Sulfoxide is Less Effective in Immersing Cryopreserved Large Pieces of Tissue: A Rabbit Hind-Limb Model.二甲基亚砜在浸泡冷冻大块组织时效果较差：兔后肢模型。
Med Sci Monit. 2019 Oct 22;25:7908-7913. doi: 10.12659/MSM.917775.
3
Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model.成功实现优化灌注和冷冻保存整个卵巢并同种异体移植，改善了卵巢早衰大鼠模型的生育能力。
J Ovarian Res. 2018 May 2;11(1):35. doi: 10.1186/s13048-018-0401-4.
4
Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant.使用 DMSO 作为冷冻保护剂，可完全防止冷冻保存的整个牛和人卵巢的冷冻损伤。
J Assist Reprod Genet. 2017 Sep;34(9):1217-1229. doi: 10.1007/s10815-017-0963-x. Epub 2017 Jun 3.
5
Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant.使用无二甲亚砜冷冻保护剂成功冷冻保存整只绵羊卵巢。
J Assist Reprod Genet. 2015 Aug;32(8):1267-75. doi: 10.1007/s10815-015-0513-3. Epub 2015 Jun 19.
本文引用的文献
1
Applicability of adult techniques for ovarian preservation to childhood cancer patients.成人技术在儿童癌症患者卵巢保存中的适用性。
J Assist Reprod Genet. 2012 Sep;29(9):985-95. doi: 10.1007/s10815-012-9821-z. Epub 2012 Jul 21.
2
Live birth after transplantation of frozen-thawed ovarian tissue after bilateral oophorectomy for benign disease.良性疾病行双侧卵巢切除术后冻融卵巢组织移植后活产。
Fertil Steril. 2012 Sep;98(3):720-5. doi: 10.1016/j.fertnstert.2012.05.017. Epub 2012 Jun 13.
3
Long-term duration of function of ovarian tissue transplants: case reports.卵巢组织移植功能的长期持续时间：病例报告。
Reprod Biomed Online. 2012 Aug;25(2):128-32. doi: 10.1016/j.rbmo.2012.03.014. Epub 2012 Apr 5.
4
Ovary cryopreservation and transplantation for fertility preservation.卵巢组织冻存与移植用于保存生育力。
Mol Hum Reprod. 2012 Feb;18(2):59-67. doi: 10.1093/molehr/gar082. Epub 2011 Dec 28.
5
Preservation of fertility in females with haematological malignancy.血液系统恶性肿瘤患者的生育力保存。
Br J Haematol. 2011 Jul;154(2):175-84. doi: 10.1111/j.1365-2141.2011.08723.x. Epub 2011 May 12.
6
Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries.冷却速率和冰核温度对整个卵巢冷冻保存的影响。
J Assist Reprod Genet. 2011 Jul;28(7):627-33. doi: 10.1007/s10815-011-9557-1. Epub 2011 Mar 23.
7
Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?带血管蒂的卵巢整体冷冻保存：玻璃化法还是传统冷冻法？
J Assist Reprod Genet. 2011 May;28(5):445-52. doi: 10.1007/s10815-011-9539-3. Epub 2011 Feb 3.
8
Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births.冻存卵巢组织自体移植后出生的儿童：13 例活产回顾。
Ann Med. 2011;43(6):437-50. doi: 10.3109/07853890.2010.546807. Epub 2011 Jan 13.
9
Ovarian function 6 years after cryopreservation and transplantation of whole sheep ovaries.绵羊卵巢组织冻存与移植后 6 年的卵巢功能
Reprod Biomed Online. 2010 Jan;20(1):48-52. doi: 10.1016/j.rbmo.2009.10.019. Epub 2009 Oct 31.
10
Time to re-think: ovarian tissue transplantation versus whole ovary transplantation.重新思考：卵巢组织移植与整个卵巢移植。
Reprod Biomed Online. 2010 Feb;20(2):171-4. doi: 10.1016/j.rbmo.2009.11.019. Epub 2009 Nov 29.
Zotero 插件