Kasicka-Jonderko Anna, Loska Dorota, Jonderko Krzysztof, Kaminska Magdalena, Błonska-Fajfrowska Barbara
Department of Basic Biomedical Science, School of Pharmacy, Medical University of Silesia, Sosnowiec, Poland.
Isotopes Environ Health Stud. 2011 Mar;47(1):34-41. doi: 10.1080/10256016.2010.549229.
It is essential to establish whether and how environmental factors affect the reliability of [(13)C]methacetin breath test ((13)C-MBT). In 12 healthy volunteers (smokers), a standard (13)C-MBT with 75 mg [(13)C]methacetin was performed twice in random order: on a control day without smoking and on another day with smoking two cigarettes antecedently. A considerable flattening of the curve of the momentary (13)C recovery within the expiratory air was observed when the (13)C-MBT was performed after smoking. The maximum of the momentary (13)C recovery, D(max), decreased from 37.20±2.58 to 25.39±2.29% dose/h (p=0.00052). Moreover, the time to reach D(max) was prolonged after cigarette smoking (26.5±3.1 vs. 16.5±1.9 min, p=0.0199). The curve of the cumulative (13)C recovery on the cigarette smoking day appeared to be shifted downwards, and statistically significant differences relative to the control situation were found between the 24th and 75th minute following [(13)C]methacetin administration. Smoking cigarettes immediately prior to the (13)C-MBT diminishes the ability of the liver to handle methacetin, and hence a possibility of such an interaction should be excluded in order to interpret the results of the test correctly.
确定环境因素是否以及如何影响[（13）C]美沙西汀呼气试验（（13）C-MBT）的可靠性至关重要。在12名健康志愿者（吸烟者）中，以随机顺序进行了两次含75毫克[（13）C]美沙西汀的标准（13）C-MBT：一次在不吸烟的对照日进行，另一次在事先吸两支烟的日子进行。在吸烟后进行（13）C-MBT时，观察到呼气中瞬时（13）C回收率曲线明显变平。瞬时（13）C回收率的最大值D（max）从37.20±2.58降至25.39±2.29%剂量/小时（p=0.00052）。此外，吸烟后达到D（max）的时间延长（26.5±3.1对16.5±1.9分钟，p=0.0199）。吸烟日累积（13）C回收率曲线似乎向下移动，在给予[（13）C]美沙西汀后的第24分钟至第75分钟之间，相对于对照情况发现了统计学上的显著差异。在（13）C-MBT之前立即吸烟会降低肝脏处理美沙西汀的能力，因此为了正确解释试验结果，应排除这种相互作用的可能性。
