Einollahi Nahid, Dashti Nasrin, Nabatchian Fariba
Department of Medical Laboratory Sciences, School of Allied Health Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Acta Med Iran. 2010 Sep-Oct;48(5):300-3.
Leptin, a circulating 16-kd polypeptide consisting of 167 amino acids, appears to be involved in the body weight homeostasis. Moreover leptin plays an important role for the reproductive system, early embryogenesis, and fat metabolism during pregnancy and puberty. Significant correlations have been found between leptin and sexual hormones, which is a cytokine and has hormonal properties. The aim of this study was to determine serum leptin levels during the menstrual cycle, and the association between serum leptin and reproductive hormones in young, healthy Iranian women. 42 healthy women volunteered for the study. They all had regular menstrual cycles, with cycle length varying between 26 and 32 days. None of them used oral contraceptives. All were of normal weight, with body mass index ( BMI) < 25 Kg/m2. Fasting blood samples were collected during the follicular phase, mid cycle and luteal phase of the menstrual cycle. FSH and LH were measured with coated tube immunoradiometric assay. Estrogen and progesterone were measured using antibody -coated tubes. Serum Leptin concentration were measured by Leptin (sandwich) ELISA. In menstruating women, serum leptin increased from 13.15+/-1.60 ng/ml in the early follicular phase to 16.57+/-1.68 ng/ml (P<0.01) at the luteal phase. Serum leptin concentration negatively correlated with LH and progesterone (P<0.05). Mean serum leptin levels correlated with body mass index (BMI) (r =0.78, P<0.001).
瘦素是一种由167个氨基酸组成的16千道尔顿循环多肽，似乎参与了体重的稳态调节。此外，瘦素在生殖系统、早期胚胎发育以及妊娠和青春期的脂肪代谢中发挥着重要作用。已发现瘦素与性激素之间存在显著相关性，瘦素是一种细胞因子，具有激素特性。本研究的目的是测定年轻健康伊朗女性月经周期中的血清瘦素水平，以及血清瘦素与生殖激素之间的关联。42名健康女性自愿参与本研究。她们的月经周期均规律，周期长度在26至32天之间。她们均未使用口服避孕药。所有人体重均正常，体重指数（BMI）<25kg/m²。在月经周期的卵泡期、周期中期和黄体期采集空腹血样。采用包被管免疫放射分析法测定促卵泡生成素（FSH）和促黄体生成素（LH）。使用抗体包被管测定雌激素和孕酮。采用瘦素（夹心）酶联免疫吸附测定法测定血清瘦素浓度。在月经女性中，血清瘦素从卵泡早期的13.15±1.60ng/ml增加到黄体期的16.57±1.68ng/ml（P<0.01）。血清瘦素浓度与LH和孕酮呈负相关（P<0.05）。血清瘦素平均水平与体重指数（BMI）相关（r=0.78，P<0.001）。
