[通过RNA分析确定血迹形成时间]
[Determination of bloodstain formation time by RNA analysis].
作者信息
Xu Yan, Jiang Wei, Ping Yuan, Bi Gang, Chen Lian-Kang, Zhou Huai-Gu
机构信息
Shanghai Key Laboratory of Crime Science Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
出版信息
Fa Yi Xue Za Zhi. 2010 Oct;26(5):340-2.
OBJECTIVE
To investigate the expression of 18S rRNA and beta-actin mRNA in bloodstain between 8 and 15 days after death and extrapolate the time of bloodstain formation.
METHODS
RNA in dried bloodstain at different times was extracted, then quantified for 18S rRNA and beta-actin mRNA by real-time RT-PCR. The bloodstain formation time was deduced based on the changes of the ratio of 18S rRNA to beta-actin mRNA at different time points.
RESULTS
The ratio of 18S rRNA to beta-actin mRNA increased gradually with time, indicating that rRNA and mRNA degraded in different rate with time. CONCLUSION; The ratio of 18S rRNA to beta-actin mRNA could be used for estimating the time of bloodstain formation in some period.
目的
研究死后8至15天血痕中18S rRNA和β-肌动蛋白mRNA的表达情况，并推断血痕形成时间。
方法
提取不同时间干燥血痕中的RNA，然后通过实时逆转录聚合酶链反应对18S rRNA和β-肌动蛋白mRNA进行定量。根据不同时间点18S rRNA与β-肌动蛋白mRNA比值的变化推断血痕形成时间。
结果
18S rRNA与β-肌动蛋白mRNA的比值随时间逐渐增加，表明rRNA和mRNA随时间以不同速率降解。结论：18S rRNA与β-肌动蛋白mRNA的比值可用于在一定时期内估计血痕形成时间。
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