[根据大鼠β-肌动蛋白mRNA稳定性估计死后间隔时间的初步研究]
[A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat].
作者信息
Xiao Jun-hui, Chen Yu-chuan, Wang Jiang-feng
机构信息
Department of forensic pathology, preliminary medical college of Sun Yat-Sen University, Guangzhou 510080, China.
出版信息
Fa Yi Xue Za Zhi. 2005 Feb;21(1):19-20.
OBJECTIVE
In order to find a new parameter to estimate the postmortem interval, beta-actin mRNA in lung and thoracic muscle of rats was detected at different time point postmortem.
METHODS
Rats were killed by neck dislocation and left in a temperature controlling system at 21 degrees C for 12 days postmortem. Total RNA in lung and thoracic muscle at different time point was extracted and beta-actin mRNA expression was examined by RT-PCR. Semi-quantification analysis of the image of electrophoresis was performed to confirm the changes of beta-actin mRNA expression.
RESULTS
beta-actin mRNA in lung of rats still could be detected at 12 days postmortem, but-disappeared in thoracic muscle at 8 days postmortem.
CONCLUSION
The expression of beta-actin mRNA in lung and thoracic muscle could be a new parameter for estimation of PMI.
目的
为寻找一种估计死亡时间间隔的新参数，在大鼠死后不同时间点检测其肺和胸肌中的β-肌动蛋白mRNA。
方法
将大鼠通过颈椎脱臼处死，并在21℃的温度控制系统中放置12天。提取不同时间点肺和胸肌中的总RNA，并通过逆转录聚合酶链反应（RT-PCR）检测β-肌动蛋白mRNA的表达。对电泳图像进行半定量分析以确认β-肌动蛋白mRNA表达的变化。
结果
大鼠死后12天仍可在肺中检测到β-肌动蛋白mRNA，但在死后8天胸肌中该mRNA消失。
结论
肺和胸肌中β-肌动蛋白mRNA的表达可能是估计死亡时间间隔的一个新参数。
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