[利用大鼠脑、心脏和肾脏中的β-肌动蛋白mRNA估计早期死后间隔时间]
[Estimation of early postmortem interval using beta-actin mRNA in rat's brain, heart and kidney].
作者信息
Liu Yue-lin, Ma Kai-Jun, Li Wen-can, Xu Hong-mei, Xue Ai-min, Shen Yi-wen, Zhou Yue-qin, Zhao Zi-qin
机构信息
Department of Forensic Medicine, Shanghai Medical College of Fudan University, Shanghai 200032, China.
出版信息
Fa Yi Xue Za Zhi. 2011 Feb;27(1):5-8.
OBJECTIVE
To explore the relationship between beta-actin mRNA degradation in SD rat's brain, heart and kidney and early postmortem interval (PMI) in order to find new markers for estimating early PMI.
METHODS
Rats were sacrificed and kept in the place at a temperature of 20 degrees C. The total RNA were extracted from the brain, heart and kidney at different PMI points. Real time RT-PCR was applied to determine beta-actin mRNA levels in total RNA and the results were given in the form of Ct values. Linear relationships between PMI and Ct values were obtained and the functions of linear regression were established.
RESULTS
The great decrease of beta-actin mRNA level were observed in the three organs. The degradation rate was obviously higher in 24 hours after death in the heart and kidney. However, there were no significant changes in the brain. The changes of Ct values and PMI showed a good linear relationship.
CONCLUSION
beta-actin mRNA in rat's brain, heart and kidney degrades obviously after death and can be used for estimating early PMI by its degradation rules.
目的
探讨SD大鼠脑、心、肾中β-肌动蛋白mRNA降解与早期死后间隔时间（PMI）的关系，以寻找估计早期PMI的新指标。
方法
处死大鼠并置于20℃环境中。在不同PMI时间点从脑、心、肾提取总RNA。应用实时RT-PCR测定总RNA中β-肌动蛋白mRNA水平，结果以Ct值形式给出。获得PMI与Ct值之间的线性关系并建立线性回归方程。
结果
在三个器官中均观察到β-肌动蛋白mRNA水平大幅下降。心脏和肾脏在死后24小时内降解速率明显更高，而脑内无显著变化。Ct值变化与PMI呈良好线性关系。
结论
大鼠脑、心、肾中的β-肌动蛋白mRNA在死后明显降解，可根据其降解规律用于估计早期PMI。
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