Capital Medical University, Beijing 100069, China.
J Tradit Chin Med. 2010 Dec;30(4):265-71. doi: 10.1016/s0254-6272(10)60054-4.
To investigate color and microvascular blood flow of the tongue in the mini-swine with immune hepatic injury.
Six Chinese mini-swine for experimental use, 3 males and 3 females, were randomly divided into two groups, normal group and model group, 3 swine in each group. The swine in the model group was administrated by injection of 5 mg/kg ConA into the vein of auricular back, once every other day, 3 times each week, for 2 weeks in total. The animal in the control group was administrated with equal volume of saline. At 9 o'clock in the morning of the 15th day of the experiment, each swine was anesthetized with intramuscular injection of 9 ml 2.5% pentobarbital sodium and 3 ml Maleate, and then picture of the tongue was taken, microvascular blood flow on the tongue and the liver was detected with a laser Doppler blood flowmeter; Blood was taken from the precaval vein. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (Tbil) and total protein (TP) were determined; Pathological changes of the liver and tongue tissues were investigated by means of HE staining; Serum TNF-alpha content was detected with ELISA assay.
In the mini-swine with immune hepatic injury induced by ConA, the tongue color showed cyanotic color, microvascular perfusion in the liver and the tongue, and partial pressure of oxygen in the tongue tissue significantly decreased; and the microcirculatory perfusion of the tongue was significantly correlated with that of the liver and the HIS color spatial value of the tongue; Serum TNF-alpha content significantly increased.
The mini-swine with immune hepatic injury induced by ConA conforms to pathological characteristics of immune hepatic injury. Formation of the cyanotic tongue is related with microcirculatory disturbance of the tongue, which can indirectly reflect hepatic microcirculatory state in the immune hepatic injury.
探讨免疫性肝损伤小型猪舌色与舌微血管血流变化。
6 头实验用小型猪，雌雄不拘，随机分为正常组和模型组，每组 3 头。模型组猪耳缘静脉注射 ConA 5mg/kg，隔 1 天 1 次，每周 3 次，共 2 周。对照组猪注射等容量生理盐水。实验第 15 天 9 时，每头猪肌肉注射 2.5%戊巴比妥钠 9ml 和马来酸 3ml 麻醉，采集舌像，应用激光多普勒血流仪检测舌微血管血流及肝血流；经颈前静脉采血，检测血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆红素（Tbil）、总蛋白（TP）；HE 染色观察肝、舌组织病理学变化；酶联免疫吸附试验（ELISA）法检测血清 TNF-α含量。
在 ConA 诱导的免疫性肝损伤小型猪中，舌色呈青紫色，肝及舌微血管灌注及舌组织氧分压明显降低，且舌微血管灌注与肝及舌 HIS 颜色空间值呈显著相关；血清 TNF-α含量明显升高。
ConA 诱导的免疫性肝损伤小型猪符合免疫性肝损伤的病理特点，青紫色舌的形成与舌微血管循环障碍有关，可间接反映免疫性肝损伤时的肝微循状态。
