Department of Neurology, The First Affiliated Hospital of Henan College of Traditional Chinese Medicine, Zhengzhou 450000, China.
J Tradit Chin Med. 2010 Dec;30(4):278-82. doi: 10.1016/s0254-6272(10)60056-8.
To explore the mechanism of Buyang Huanwu Tang (Decoction Invigorating Yang for Recuperation) combined with bone marrow mesenchymal stem cells (MSCs) transplantation in protecting nerves of cerebral ischemic injury.
Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang was administrated. Vascular endothelial growth factor (VEGF) and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method.
VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group (P < 0.05), and with the most strongest effect in the combination group.
Buyang Huanwu Tang combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression.
探讨补阳还五汤联合骨髓间充质干细胞（MSCs）移植对脑缺血损伤神经保护作用的机制。
采用改良 Zea-Longa 线栓法建立大脑局部缺血再灌注大鼠模型，MSCs 经尾静脉注射，同时给予补阳还五汤灌胃。采用免疫组织化学染色法检测脑缺血再灌注大鼠脑缺血侧 VEGF 和 Ki-67 的表达。
MSCs 组和联合组 VEGF 和 Ki-67 的表达明显上调，与模型组和假手术组比较差异有统计学意义（P < 0.05），且以联合组作用最强。
补阳还五汤联合 MSCs 移植可能通过上调 VEGF 和 Ki-67 的表达来修复损伤的血管和病变组织。
