Max Planck Institute for Polymer Research, 55128 Mainz, Germany.
J Am Chem Soc. 2011 Feb 23;133(7):2025-7. doi: 10.1021/ja1092002. Epub 2011 Feb 2.
The rupture force of a split (bipartite) aptamer that forms binding pockets for adenosine monophosphate (AMP) was measured by atomic force spectroscopy. Changes in the rupture force were observed in the presence of AMP, while this effect was absent when mutant aptamers or inosine were used. Thus, changes in the rupture force were a direct consequence of specific binding of AMP to the split aptamer. The split aptamer concept allowed the detection of nonlabeled AMP and enabled us to determine the dissociation constant on a single-molecule level.
通过原子力光谱法测量了形成单磷酸腺苷（AMP）结合口袋的分裂（二分体）适体的断裂力。在存在 AMP 的情况下观察到断裂力的变化，而当使用突变适体或肌苷时则没有这种效应。因此，断裂力的变化是 AMP 与分裂适体特异性结合的直接结果。分裂适体的概念允许检测未标记的 AMP，并使我们能够在单分子水平上确定离解常数。
