Abid-Essefi S, Zaied C, Bouaziz C, Salem I Ben, Kaderi R, Bacha H
Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Monastir University, Rue Avicenne, 5019 Monastir, Tunisia.
Exp Toxicol Pathol. 2012 Nov;64(7-8):689-95. doi: 10.1016/j.etp.2010.12.012. Epub 2011 Feb 1.
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. It has been implicated in several mycotoxicosis in farm animals and in humans. Several reports suggest that oxidative damage seems to be a key determinant of ZEN induced toxicity in vitro and in vivo. The aim of the current study was to evaluate the protective effects of aqueous extract of Allium sativum (AEA), against ZEN-induced cytotoxicity, reactive oxygen species (ROS) generation and DNA fragmentation in cultured Vero cells. Indeed, cytotoxicity effects were studied using MTT viability assay, ROS generation measurement and catalase activity induction. To check whether the oxidative stress induction was associated to DNA lesions, we looked for DNA fragmentation using Comet test. Our results indicated that ZEN induced several toxic effects and significant alterations mediated by oxidative stress mechanism. Treatment by ZEN combined to the lowest dose of AEA (250 μg/ml) showed a significant reduction of ZEN induced damages for all tested markers and a noticeable reduction of DNA fragmentation. It could be concluded that A. sativum aqueous extracts is effective in the protection against ZEN hazards. This could be relevant, particularly with the emergent demand for natural products which may counteract the detrimental toxic effects mediated by oxidative stress process and therefore prevents multiple human diseases.
玉米赤霉烯酮（ZEN）是由谷物和农产品中的几种镰刀菌产生的一种非甾体雌激素类霉菌毒素。它与农场动物和人类的多种霉菌毒素中毒有关。几份报告表明，氧化损伤似乎是ZEN在体外和体内诱导毒性的关键决定因素。本研究的目的是评估大蒜水提取物（AEA）对ZEN诱导的培养Vero细胞的细胞毒性、活性氧（ROS）生成和DNA片段化的保护作用。事实上，使用MTT活力测定、ROS生成测量和过氧化氢酶活性诱导来研究细胞毒性作用。为了检查氧化应激诱导是否与DNA损伤有关，我们使用彗星试验寻找DNA片段化。我们的结果表明，ZEN诱导了几种毒性作用以及由氧化应激机制介导的显著改变。ZEN与最低剂量的AEA（250μg/ml）联合处理显示，所有测试指标的ZEN诱导损伤均显著降低，DNA片段化也明显减少。可以得出结论，大蒜水提取物对预防ZEN危害有效。这可能具有相关性，特别是鉴于对天然产物的迫切需求，这些天然产物可能抵消由氧化应激过程介导的有害毒性作用，从而预防多种人类疾病。
