Degré M, Bukholm G
Frues Bakteriologiske Institutt, Rikshospitalet, University of Oslo, Norway.
J Biol Regul Homeost Agents. 1990 Oct-Dec;4(4):157-61.
Mice, inoculated with Salmonella typhimurium, either intraperitoneally (i.p.) or intragastrically (i.g.), developed a systemic infection with a high death rate within a few days after inoculation. Pretreatment of the mice with moderate concentrations of i.p. administered TNF-alpha 24 h before the administration of bacteria reduced the establishment of intracellular infection in the intestinal epithelial cells, and development of bacteremia. The mortality rate was reduced, and the survival time was extended by the same treatment. This effect of TNF-alpha was more pronounced against i.g. than against i.p. inoculated bacteria. The effect was dose dependent, thus concentrations above or below the optimal dose had less effect. No synergistic effect was seen if TNF-alpha was given in combination with interferon-gamma. These results indicate, that TNF-alpha may have a physiological effect in the host defence against facultatively intracellular Gram-negative bacteria.
通过腹腔内（i.p.）或胃内（i.g.）接种鼠伤寒沙门氏菌的小鼠，在接种后几天内会发生全身性感染，死亡率很高。在给细菌前24小时，用中等浓度的腹腔注射肿瘤坏死因子-α（TNF-α）对小鼠进行预处理，可减少肠道上皮细胞内细胞感染的建立以及菌血症的发生。相同的处理降低了死亡率，并延长了存活时间。TNF-α对胃内接种细菌的作用比对腹腔内接种细菌的作用更明显。该作用具有剂量依赖性，因此高于或低于最佳剂量的浓度效果较差。如果将TNF-α与干扰素-γ联合使用，则未观察到协同作用。这些结果表明，TNF-α在宿主抵御兼性细胞内革兰氏阴性菌的防御中可能具有生理作用。
