Bocci V, Paulesu L
Istituto di Fisiologia Generale, Università di Siena, Italy.
Haematologica. 1990 Nov-Dec;75(6):510-5.
In this study we have investigated the effects of ozone on human blood, as well as on resuspended buffy coats and Ficoll-purified mononuclear cells. Samples were exposed at different ozone concentrations (from 2.2 micrograms to 108 micrograms/ml) for 30 sec and then incubated for different times at 37 degrees C in a 95% air-5% CO2 humidified atmosphere. Supernatants were collected and frozen at-20 degrees C until tested for interferon (IFN) activity. We have determined that the ozone concentration is critical for lymphokine induction. In fact, while low concentrations (2.2 micrograms/ml) are effective in lymphocytes, they do not induce IFN in either whole or diluted (1:1) human blood, or resuspended buffy coats. In such cases levels as high as 42 micrograms/ml are required. On the other hand, a very high ozone concentration (108 micrograms/ml) is not effective and probably toxic. Maximal IFN production occurs 72-96 h after ozone exposure, and the kinetics of IFN release is similar to that after Staphylococcal Enterotoxin B addition. Because ozonization of blood is a medical procedure followed in several countries for treatment of viral diseases, this study can open a new field of investigation that may yield useful results both in biological and practical terms.
在本研究中，我们调查了臭氧对人体血液以及重悬的血沉棕黄层和经Ficoll分离的单核细胞的影响。将样本暴露于不同臭氧浓度（2.2微克至108微克/毫升）下30秒，然后在37摄氏度、95%空气-5%二氧化碳的湿润气氛中孵育不同时间。收集上清液并在-20摄氏度下冷冻，直至检测干扰素（IFN）活性。我们已经确定臭氧浓度对淋巴因子诱导至关重要。事实上，虽然低浓度（2.2微克/毫升）对淋巴细胞有效，但它们在全血或稀释（1:1）的人体血液或重悬的血沉棕黄层中均不诱导IFN产生。在这种情况下，需要高达42微克/毫升的浓度。另一方面，非常高的臭氧浓度（108微克/毫升）无效且可能有毒。臭氧暴露后72 - 96小时IFN产生达到最大值，IFN释放动力学与添加葡萄球菌肠毒素B后的情况相似。由于血液臭氧处理是一些国家用于治疗病毒性疾病的一种医疗程序，本研究可以开辟一个新的研究领域，可能在生物学和实际应用方面都产生有用的结果。
