Paulesu L, Luzzi E, Bocci V
Institute of General Physiology, University of Siena, Italy.
Lymphokine Cytokine Res. 1991 Oct;10(5):409-12.
The effect of ozone as a probable inducer of tumor necrosis factor (TNF-alpha) has been investigated on human blood and on Ficoll-purified blood mononuclear cells (PBMC). Samples were exposed at different ozone concentrations ranging from 2.2 to 108 micrograms/ml and incubated at 37 degrees C in an 95% air-5% CO2 atmosphere. At predetermined times, all cell supernatants were tested for TNF activity and some PBMC cultures were examined for DNA synthesis. We have shown that ozone concentration is critical in terms of TNF production and of cell mitogenesis and that, owing to the presence of erythrocytes, higher ozone concentrations are required to be effective in blood than in PBMC. Because ozonization of blood is a procedure followed in several European countries for the treatment of viral diseases and tumors, the release of factors with antiviral and immunomodulatory activities by leukocytes may explain the mechanism of action of ozone and of autohemotherapy.
臭氧作为肿瘤坏死因子（TNF-α）可能诱导剂的作用已在人体血液和经Ficoll纯化的血液单核细胞（PBMC）上进行了研究。样本暴露于2.2至108微克/毫升的不同臭氧浓度下，并在37℃、95%空气-5%二氧化碳的气氛中孵育。在预定时间，检测所有细胞上清液的TNF活性，并检查一些PBMC培养物的DNA合成情况。我们已经表明，臭氧浓度对于TNF的产生和细胞有丝分裂至关重要，并且由于红细胞的存在，与PBMC相比，血液中需要更高的臭氧浓度才能产生效果。由于在几个欧洲国家血液臭氧疗法是用于治疗病毒性疾病和肿瘤的一种方法，白细胞释放具有抗病毒和免疫调节活性的因子可能解释了臭氧和自血疗法的作用机制。
