枯草芽孢杆菌孢子中一种孢子萌发蛋白GerAB的免疫电子显微镜定位
Immunoelectron microscopic localization of one of the spore germination proteins, GerAB, in Bacillus subtilis spores.
作者信息
Sakae Y, Yasuda Y, Tochikubo K
机构信息
Department of Microbiology, Nagoya City University Medical School, Japan.
出版信息
J Bacteriol. 1995 Nov;177(21):6294-6. doi: 10.1128/jb.177.21.6294-6296.1995.
Abstract
Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was investigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immunoglobulin G complex. Immunogold particles were visualized in the boundary region between the cortex and coat of dormant spores, and they were broadly dispersed into the cortex region after germination.
摘要
通过使用抗肽（GerAB的61至80位氨基酸残基）抗血清和胶体金-免疫球蛋白G复合物的免疫电子显微镜技术，研究了枯草芽孢杆菌孢子中与L-丙氨酸引发的萌发相关的蛋白质之一GerAB的超微结构定位。在休眠孢子的皮层和外壳之间的边界区域观察到免疫金颗粒，萌发后它们广泛分散到皮层区域。
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