Zhang Ronggui, Chen Wenjun, Zhang Weili, Jiang Qing, Liu Chuan, Lin Yanjun, Hu Zili, Yu Shengjie, Xu Guangyong
Department of Urology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Genet Test Mol Biomarkers. 2011 May;15(5):337-41. doi: 10.1089/gtmb.2010.0230. Epub 2011 Feb 3.
p53 is one of the most widely investigated molecular markers in bladder cancer and its polymorphisms have been related to individual cancer risks. The objective of this study was to explore the association of p53 codon 72 polymorphism with susceptibility and clinicopathologic characteristics of bladder cancer in a Chinese population.
We investigated the impact of p53 codon 72 polymorphism in a hospital-based case-control study of bladder cancer. We tested peripheral blood samples from 120 patients with bladder cancer and 120 healthy individuals of similar age and from the same geographical region. The polymorphisms were analyzed using polymerase chain reaction-restriction fragment length polymorphism assay.
There was an association between smoking status and bladder cancer (odds ratio [OR] = 2.25; 95% confidence interval [95% CI] = 1.31, 3.87; p = 0.003). Patients with bladder cancer had a significantly lower frequency of Arg/Arg (OR = 0.53; 95% CI = 0.31, 0.89; p = 0.02) and Arg allele (OR = 0.66; 95% CI = 0.45, 0.95; p = 0.03) than controls. Patients with invasive bladder cancer had a significantly lower frequency of Arg/Arg (OR = 0.29; 95% CI = 0.10, 0.88; p = 0.03) than those with superficial bladder cancer. When stratifying by the grade and histological type of bladder cancer, we found no statistical association.
These data suggest that the p53 codon 72 Arg/Arg genotype and Arg allele are associated with a lower risk of bladder cancer in Chinese population.
p53是膀胱癌研究最为广泛的分子标志物之一，其多态性与个体患癌风险相关。本研究旨在探讨中国人群中p53密码子72多态性与膀胱癌易感性及临床病理特征的关系。
我们在一项基于医院的膀胱癌病例对照研究中，调查了p53密码子72多态性的影响。我们检测了120例膀胱癌患者以及120例年龄相仿且来自同一地理区域的健康个体的外周血样本。采用聚合酶链反应-限制性片段长度多态性分析方法对多态性进行分析。
吸烟状况与膀胱癌之间存在关联（优势比[OR]=2.25；95%置信区间[95%CI]=1.31, 3.87；p=0.003）。膀胱癌患者中Arg/Arg基因型（OR=0.53；95%CI=0.31, 0.89；p=0.02）和Arg等位基因（OR=0.66；95%CI=0.45, 0.95；p=0.03）的频率显著低于对照组。浸润性膀胱癌患者中Arg/Arg基因型的频率（OR=0.29；95%CI=0.10, 0.88；p=0.03）显著低于浅表性膀胱癌患者。按膀胱癌的分级和组织学类型进行分层时，我们未发现统计学关联。
这些数据表明，在中国人群中，p53密码子72的Arg/Arg基因型和Arg等位基因与较低的膀胱癌风险相关。
