Food Research & Development Center, Samsung Everland Inc., Giheung-gu, Yongin, South Korea.
Lett Appl Microbiol. 2011 Apr;52(4):399-405. doi: 10.1111/j.1472-765X.2011.03018.x. Epub 2011 Feb 24.
Salmonella spp. are an important cause of food-borne infections throughout world, and the availability of rapid and simple detection techniques is critical for the food industry. Salmonella enterica serovars Enteritidis and Typhimurium cause the majority of human gastroenteritis infections, and there are a reported 40,000 cases of salmonellosis in the United States each year.
A novel rapid and simple isothermal target and probe amplification (iTPA) assay that rapidly amplifies target DNA (Salmonella invA gene) using a FRET-based signal probe in an isothermal environment was developed for detection Salmonella spp. in pre-enriched food samples. The assay was able to specifically detect all of 10 Salmonella spp. strains without detecting 40 non-Salmonella strains. The detection limit was 4 x 10¹ CFU per assay. The iTPA assay detected at an initial inoculum level of <10 CFU in the pre-enriched food samples (egg yolk, chicken breast and peanut butter).
This detection system requires only a water bath and a fluorometer and has great potential for use as a hand-held device or point-of-care-testing diagnostics. The iTPA assay is sensitive and specific and has potential for rapid screening of Salmonella spp. by food industry.
沙门氏菌属是世界各地食源性感染的重要原因，快速简便的检测技术的可用性对食品工业至关重要。肠炎沙门氏菌血清型肠炎和鼠伤寒沙门氏菌引起大多数人类肠胃炎感染，据报道，美国每年有 4 万例沙门氏菌病。
开发了一种新颖的快速简便等温靶标和探针扩增（iTPA）检测方法，该方法在等温环境中使用基于 FRET 的信号探针快速扩增靶 DNA（沙门氏菌 invA 基因），用于检测预富集食品样品中的沙门氏菌属。该检测方法能够特异性地检测到所有 10 株沙门氏菌，而不会检测到 40 株非沙门氏菌。检测限为每个检测 4 x 10¹ CFU。iTPA 检测方法在预富集食品样品（蛋黄、鸡胸肉和花生酱）中的初始接种水平<10 CFU 时即可检测到。
该检测系统仅需水浴和荧光计，非常适合用作手持式设备或即时检测诊断。iTPA 检测方法具有高度的敏感性和特异性，有可能通过食品工业快速筛选沙门氏菌。
