Guo Jiubiao, Chan Edward W C, Chen Sheng, Zeng Zhenling
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research InstituteShenzhen, China.
Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research InstituteShenzhen, China; State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic UniversityHung Hom, Hong Kong.
Front Microbiol. 2017 Jan 17;8:8. doi: 10.3389/fmicb.2017.00008. eCollection 2017.
A novel, rapid and simple fluorescence resonance energy transfer (FRET) based specific gene, , detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the specific gene could be sensitively and specifically detected with a limit of detection (LOD) of ∼4 nM of the gene in 20 min. The developed system has the potential to be used for detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens.
开发了一种基于荧光共振能量转移（FRET）的新型、快速且简单的特定基因检测系统，其中量子点（QDs）和氧化石墨烯（GO）分别作为荧光供体和猝灭剂。通过测量荧光强度信号，可在20分钟内灵敏且特异地检测该特定基因，检测限（LOD）约为该基因的4 nM。所开发的系统有潜力用于食品和环境样品的检测，并进一步发展成为检测其他细菌病原体的平台。
