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已发表 PCR 引物在检测人类鼻病毒感染中的应用。

Usefulness of published PCR primers in detecting human rhinovirus infection.

机构信息

The University of Queensland, Brisbane, Queensland, Australia.

出版信息

Emerg Infect Dis. 2011 Feb;17(2):296-8. doi: 10.3201/eid1702.101123.

DOI:10.3201/eid1702.101123
PMID:21291610
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3204776/
Abstract

We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.

摘要

我们对已发表的人鼻病毒（HRV）特异性 PCR 引物对的相对敏感性进行了初步比较，改变了寡核苷酸和退火温度。在 2 组基因分型临床标本中，没有一对引物能够检测到所有 HRV；为了准确描述 HRV 流行病学，需要进行多次 PCR。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e7/3204776/721d984ad436/10-1123-F.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e7/3204776/721d984ad436/10-1123-F.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e7/3204776/721d984ad436/10-1123-F.jpg

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