Department of Virology, Centre Pasteur of Cameroon, 451 Rue 2005, P.O. Box 1274, Yaoundé, Cameroon.
Department of Virology, Centre Pasteur of Cameroon, 451 Rue 2005, P.O. Box 1274, Yaoundé, Cameroon; Department of Microbiology, Faculty of Sciences, University of Yaoundé I, P.O. Box 337, Yaoundé, Cameroon.
Diagn Microbiol Infect Dis. 2019 Jul;94(3):236-242. doi: 10.1016/j.diagmicrobio.2019.01.007. Epub 2019 Jan 16.
This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test.
A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.
The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD® Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51).
Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.
本研究比较了两种不同的实时逆转录聚合酶链反应（rRT-PCR）方法对 14 种常见呼吸道病毒的检测效果：美国疾病控制与预防中心（CDC）开发的内部单重测试和市售的 Fast Track Diagnostic（FTD®）呼吸道病原体 33 多重测试。
使用 CDC 单重 rRT-PCR 和 FTD®呼吸道病原体 33 多重检测法对 217 份鼻咽拭子进行分析，以检测 14 种呼吸道病毒。
结果显示，179/217（82.5%）份样本用单重方法呈阳性，183/217（84.3%）份样本用 FTD®呼吸道病原体 33 多重检测法呈阳性。除副流感病毒 4（k=0.24）和乙型流感病毒（k=0.51）外，所有病毒（k>0.6）的一致性均为优秀或满意。
尽管 FTD 多重试剂盒比单重检测法昂贵，但 FTD 试剂盒在更短的时间内快速得出结果，提高了诊断效率。
