Wintour E M, Clemons G, Butkus A, Horvath A, Moritz K, Towstoless M K
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Australia.
J Dev Physiol. 1990 Nov;14(5):259-65.
This study examines the hypotheses that (i) erythropoietin (the hormone responsible for red blood cell production) is higher in the fetus (at low PO2) than in the neonate (at high PO2); and (ii) that the level of erythropoietin in the neonate is influenced by the presence of high oxygen affinity haemoglobin. Haematocrit (PCV), PO2 and plasma immunoreactive erythropoietin were measured in 4 chronically cannulated fetal sheep (120 days to birth, n = 22) and in 7 neonatal lambs until 233 days post-conception (75-85 days after birth, n = 83). The percentage of globin chains (alpha, gamma, beta A, beta B, beta C) was quantitated by gel electrophoresis. Plasma erythropoietin values, in 4 fetuses were 9.8 +/- 1.3 mU/ml at 120-132 days of gestation, declined significantly (P less than 0.01) to 5.2 +/- 0.4 at 133 days until birth, then increased significantly (P less than 0.001) to 24.2 +/- 5.5(5), 26.3 +/- 7.3 (6), and 24.8 +/- 8.5 (6), respectively in weeks 1, 2 and 3 of postnatal life. By weeks 5-8 the erythropoietin was 13 +/- 0.6 (4) mU/ml. PO2 was 17.4 +/- 0.9 mmHg before birth and 88.0 +/- 10.7 in the first week after birth. PCV was constant until three weeks after birth and then declined. Fetal haemoglobin had virtually disappeared from the circulation by 166 days (3 weeks after birth); in the 4 heterozygotes (beta A beta B) beta C was expressed transiently, with a maximum value of 4%, whilst in the homozygote lamb (beta A beta A) the maximum beta C was 12%.(ABSTRACT TRUNCATED AT 250 WORDS)
（i）促红细胞生成素（负责红细胞生成的激素）在胎儿（低氧分压）中的水平高于新生儿（高氧分压）；以及（ii）新生儿促红细胞生成素水平受高氧亲和力血红蛋白存在的影响。对4只长期插管的胎羊（孕期120天至出生，n = 22）和7只新生羔羊直至受孕后233天（出生后75 - 85天，n = 83）测量了血细胞比容（PCV）、氧分压（PO2）和血浆免疫反应性促红细胞生成素。通过凝胶电泳对珠蛋白链（α、γ、βA、βB、βC）的百分比进行定量。4只胎羊在妊娠120 - 132天时血浆促红细胞生成素值为9.8±1.3 mU/ml，在133天直至出生时显著下降（P＜0.01）至5.2±0.4，然后在出生后第1、2和3周分别显著升高（P＜0.001）至24.2±5.5（5）、26.3±7.3（6）和24.8±8.5（6）。到第5 - 8周时促红细胞生成素为13±0.6（4）mU/ml。出生前氧分压为17.4±0.9 mmHg，出生后第一周为88.0±10.7。血细胞比容在出生后三周内保持恒定，然后下降。胎儿血红蛋白在166天（出生后3周）时几乎从循环中消失；在4只杂合子（βAβB）中，βC短暂表达，最大值为4%，而在纯合子羔羊（βAβA）中，βC的最大值为12%。（摘要截断于250字）
