Kim Jin-Woo, Choi Kyung-Hee, Yun Jeong-Ho, Jung Ui-Won, Kim Chang-Sung, Choi Seong-Ho, Cho Kyoo-Sung
Research Institute for Periodontal Regeneration, Department of Periodontology, College of Dentistry, Yonsei University, Seodaemun-gu, Seoul, South Korea.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Sep;112(3):298-306. doi: 10.1016/j.tripleo.2010.10.025. Epub 2011 Feb 3.
The objective of this study was to evaluate bone formation in rat calvarial defects after surgical implantation of block or particulated biphasic calcium phosphate (BCP) lyophilized with Escherichia coli-derived recombinant human bone morphogenetic protein 2 (ErhBMP-2). Critical-size calvarial osteotomy defects were created in 5 groups of Sprague-Dawley rats. Each group received one of the following: 1) sham surgery control; 2) biphasic calcium phosphate particles (CPP); 3) biphasic calcium phosphate block (CPB); 4) ErhBMP-2-coated CPP; or 5) ErhBMP-2-coated CPB. ErhBMP was coated on BCP by a stepwise lyophilizing protocol. The new bone formation was significantly greater in ErhBMP-2-treated groups compared with the untreated group. In particular, the ErhBMP-2/CPB group showed stability of augmented areas during the period of healing, due to relevant space-providing capacity. Thus, it can be concluded that CPP and CPB lyophilized with ErhBMP-2 enhance the formation of new bone, and CPB appears to be a suitable carrier for ErhBMP-2 in which a 3-dimensional structural integrity is an important consideration factor.
本研究的目的是评估在手术植入用大肠杆菌衍生的重组人骨形态发生蛋白2（ErhBMP-2）冻干的块状或颗粒状双相磷酸钙（BCP）后，大鼠颅骨缺损处的骨形成情况。在5组Sprague-Dawley大鼠中制造临界大小的颅骨截骨缺损。每组接受以下之一：1）假手术对照；2）双相磷酸钙颗粒（CPP）；3）双相磷酸钙块（CPB）；4）ErhBMP-2包被的CPP；或5）ErhBMP-2包被的CPB。通过逐步冻干方案将ErhBMP包被在BCP上。与未处理组相比，ErhBMP-2处理组的新骨形成明显更多。特别是，由于相关的空间提供能力，ErhBMP-2/CPB组在愈合期间显示出增大区域的稳定性。因此，可以得出结论，用ErhBMP-2冻干的CPP和CPB可增强新骨的形成，并且CPB似乎是ErhBMP-2的合适载体，其中三维结构完整性是一个重要的考虑因素。
