Shin Y S, Seo J Y, Oh S H, Kim J H, Kim S T, Park Y B, Moon H S
Department of Conservative Dentistry, Oral Science Research Center, Yonsei University College of Dentistry, Seoul, Korea.
Oral Dis. 2014 Apr;20(3):281-7. doi: 10.1111/odi.12109. Epub 2013 May 7.
The purpose was to evaluate the effect of Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2)-/epigallocatechin-3-gallate (EGCG)-coated biphasic calcium phosphate (BCP) and titanium barrier membrane on dehiscence defects in dogs.
In five mongrel dogs, the dehiscence bony defects around dental implants were surgically created and in total three implants were placed at edentulous ridge of which teeth had been extracted 12 weeks before. For the control group, BCP was applied to the dehiscence defect. For experimental groups, ErhBMP-2-coated BCP and ErhBMP-2-/EGCG-coated BCP were applied. The newly designed titanium barrier membrane was used to apply all the defects. The defects were evaluated histologically and histometrically after 12 weeks. The comparative statistics of the groups were obtained through Kruskal-Wallis test.
In bone-to-implant contact (BIC), bone density (BD), bone regeneration height (BRH), and bone mineralization apposition rate (BMAR), differences among groups were not found. ErhBMP-2/EGCG group appeared to have higher value. In fluorescence analysis, bone remodeling around graft material was more active in the ErhBMP-2/EGCG group.
Within the limit of this study, it is reasonable to assume that BMP-2-/EGCG-coated biphasic BCP and the newly designed titanium membrane were more beneficial in dehiscence defect healing with increased bone remodeling.
评估大肠杆菌衍生的重组人骨形态发生蛋白-2（ErhBMP-2）/表没食子儿茶素-3-没食子酸酯（EGCG）包被的双相磷酸钙（BCP）和钛屏障膜对犬类骨缺损的影响。
在5只杂种犬中，通过手术制造牙种植体周围的骨缺损，在已拔牙12周的无牙牙槽嵴处共植入3颗种植体。对照组在骨缺损处应用BCP。实验组分别应用ErhBMP-2包被的BCP和ErhBMP-2/EGCG包被的BCP。所有缺损均使用新设计的钛屏障膜。12周后对缺损进行组织学和组织计量学评估。通过Kruskal-Wallis检验获得各组的比较统计数据。
在骨与种植体接触（BIC）、骨密度（BD）、骨再生高度（BRH）和骨矿化沉积率（BMAR）方面，各组之间未发现差异。ErhBMP-2/EGCG组似乎具有更高的值。在荧光分析中，ErhBMP-2/EGCG组移植物周围的骨重塑更活跃。
在本研究范围内，可以合理推测BMP-2/EGCG包被的双相BCP和新设计的钛膜在促进骨缺损愈合及增加骨重塑方面更有益。
