低剂量大肠杆菌衍生的重组人骨形态发生蛋白-2与双相磷酸钙载体在兔颅骨缺损模型中的骨再生疗效
Bone Regenerative Efficacy of Limited-Dose Escherichia Coli-Derived rhBMP-2 With Biphasic Calcium Phosphate Carrier in Rabbit Calvarial Defect Model.
作者信息
You Hoon, Yoon So-Ra, Lim Hyun-Chang, Lee Jung-Seok, Jung Ui-Won, Choi Seong-Ho
机构信息
*Post-Graduate, Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, South Korea. †Clinical Assistant Professor, Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, South Korea. ‡Clinical Assistant Professor, Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, South Korea. §Associate Professor, Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, South Korea. ¶Professor, Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, South Korea.
出版信息
Implant Dent. 2016 Feb;25(1):16-23. doi: 10.1097/ID.0000000000000364.
PURPOSE
The aim of this study was to characterize the healing in rabbit calvarial bone defects after delivery of limited-dose (1.5 μg) Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), and evaluate biphasic calcium phosphate (BCP) as a carrier.
MATERIALS AND METHODS
Four 8-mm diameter circular calvarial bone defects were made in 16 rabbits and filled with one of the following: (1) nothing, (2) BCP alone, (3) ErhBMP-2-loaded absorbable collagen sponge (ACS), or (4) ErhBMP-2-loaded BCP. The animals were allowed to heal for either 2 or 8 weeks and were evaluated in clinical, microcomputed tomographic, histological, and histomorphometric analyses.
RESULTS
Microcomputed tomography revealed extensive new bone formation in both of the limited-dose ErhBMP-2-loaded groups. However, bony collapse of the upper defect borders was found in the ErhBMP-2-loaded ACS group. Histomorphometric examination revealed significantly greater new bone formation at 8 weeks than at 2 weeks in all 4 groups (P < 0.05). Both new bone formation and the size of the augmented area differed significantly between the ErhBMP-2-loaded BCP group (6.88 ± 0.74 and 19.62 ± 0.77) and the ErhBMP-2-loaded ACS group (3.04 ± 0.27 and 5.41 ± 0.43) at 8 weeks of healing.
CONCLUSION
ErhBMP-2 promotes bone regeneration in rabbit calvarial defects, even at a limited dose (1.5 μg). The results of this study suggest that BCP is the more efficient carrier for this protein than ACS.
目的
本研究旨在描述在递送有限剂量（1.5μg）大肠杆菌衍生的重组人骨形态发生蛋白-2（ErhBMP-2）后兔颅骨骨缺损的愈合情况，并评估双相磷酸钙（BCP）作为载体的效果。
材料与方法
在16只兔子的颅骨上制作4个直径8mm的圆形骨缺损，并填充以下物质之一：（1）无，（2）单独的BCP，（3）负载ErhBMP-2的可吸收胶原海绵（ACS），或（4）负载ErhBMP-2的BCP。让动物愈合2周或8周，并进行临床、微型计算机断层扫描、组织学和组织形态计量学分析。
结果
微型计算机断层扫描显示，两个负载有限剂量ErhBMP-2的组均有广泛的新骨形成。然而，在负载ErhBMP-2的ACS组中发现了上缺损边界的骨塌陷。组织形态计量学检查显示，所有4组在8周时的新骨形成均明显多于2周时（P<0.05）。在愈合8周时，负载ErhBMP-2的BCP组（6.88±0.74和19.62±0.77）与负载ErhBMP-2的ACS组（3.04±0.27和5.41±0.43）之间的新骨形成和增大区域大小均存在显著差异。
结论
即使是有限剂量（1.5μg）的ErhBMP-2也能促进兔颅骨缺损的骨再生。本研究结果表明，对于这种蛋白质，BCP是比ACS更有效的载体。
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