鉴定和表征影响孢子形成和抗生素产生的链霉菌 globisporus 1912 调节基因 lndYR。
Identification and characterization of the Streptomyces globisporus 1912 regulatory gene lndYR that affects sporulation and antibiotic production.
机构信息
Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
Department of Microbiology, Niigata University of Pharmacy and Applied Life Sciences, Higashijima 265-1, 956-8603 Niigata, Japan.
出版信息
Microbiology (Reading). 2011 Apr;157(Pt 4):1240-1249. doi: 10.1099/mic.0.045088-0. Epub 2011 Feb 3.
Abstract
Here, we report the identification and functional characterization of the Streptomyces globisporus 1912 gene lndYR, which encodes a GntR-like regulator of the YtrA subfamily. Disruption of lndYR arrested sporulation and antibiotic production in S. globisporus. The results of in vivo and in vitro studies revealed that the ABC transporter genes lndW-lndW2 are targets of LndYR repressive action. In Streptomyces coelicolor M145, lndYR overexpression caused a significant increase in the amount of extracellular actinorhodin. We suggest that lndYR controls the transcription of transport system genes in response to an as-yet-unidentified signal. Features that distinguish lndYR-based regulation from other known regulators are discussed.
摘要
在这里，我们报告了链霉菌球孢 1912 基因 lndYR 的鉴定和功能表征，该基因编码 YtrA 亚家族的 GntR 样调节剂。lndYR 的缺失导致链霉菌球孢的孢子形成和抗生素产生停滞。体内和体外研究的结果表明，ABC 转运基因 lndW-lndW2 是 LndYR 抑制作用的靶标。在变铅青链霉菌 M145 中，lndYR 的过表达导致细胞外放线紫红素的含量显著增加。我们认为，lndYR 响应尚未确定的信号控制运输系统基因的转录。讨论了区分基于 lndYR 的调节与其他已知调节剂的特征。
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