Department of Microbiology, Faculty of Medicine, Health Science Center, Kuwait University, Kuwait.
Department of Infection Control, Farwaniya Hospital, Ministry of Health, Kuwait.
J Med Microbiol. 2011 May;60(Pt 5):600-604. doi: 10.1099/jmm.0.028381-0. Epub 2011 Feb 3.
A total of 29 meticillin-resistant Staphylococcus aureus (MRSA) isolates were obtained from 15 neonates and three healthcare workers (HCWs) in a neonatal intensive care unit (NICU) and special care baby unit (SCBU) and four patients in a medical ward of a Kuwait hospital between 10 and 30 April 2007. The isolates were characterized using antibiogram results, coagulase gene RFLP (coa-RFLP), PFGE, staphylococcal cassette chromosome mec (SCCmec) typing and multilocus sequence typing (MLST). All isolates were assessed for the carriage of Panton-Valentine leukocidin (PVL) and arginine catabolic mobile element (ACME) genes. The isolates belonged to three SCCmec types, six coa-RFLP types, six pulsotypes and six sequence types. One isolate was positive for PVL. None were positive for ACME. All MRSA isolates from the 15 neonates were phenotypically and genetically different from the MRSA isolates obtained from HCWs and those from patients in other wards. They were resistant to gentamicin, kanamycin and fusidic acid, had identical coa-RFLP and PFGE patterns, carried the type V SCCmec element and belonged to MLST sequence type ST97. The results showed the transmission of a rare clone of community-associated MRSA belonging to ST97 with the SCCmec-V genotype among neonates in a NICU and SCBU.
2007 年 4 月 10 日至 30 日期间，从科威特一家医院的新生儿重症监护病房（NICU）和特别护理婴儿病房（SCBU）的 15 名新生儿和 3 名医护人员以及 4 名病房患者中分离出 29 株耐甲氧西林金黄色葡萄球菌（MRSA）。使用抗生素谱结果、凝固酶基因 RFLP（coa-RFLP）、PFGE、葡萄球菌盒染色体 mec（SCCmec）分型和多位点序列分型（MLST）对分离株进行了特征分析。所有分离株均评估了携带杀白细胞素 Panton-Valentine（PVL）和精氨酸代谢移动元件（ACME）基因的情况。分离株属于三种 SCCmec 类型、六种 coa-RFLP 类型、六种脉冲类型和六种序列类型。有一个分离株对 PVL 呈阳性。没有分离株对 ACME 呈阳性。从 15 名新生儿中分离出的所有 MRSA 分离株与从医护人员和其他病房患者中分离出的 MRSA 分离株在表型和遗传上均不同。它们对庆大霉素、卡那霉素和夫西地酸具有耐药性，具有相同的 coa-RFLP 和 PFGE 模式，携带类型 V SCCmec 元件，属于 MLST 序列类型 ST97。结果表明，属于 ST97 的罕见社区相关 MRSA 克隆通过 SCCmec-V 基因型在 NICU 和 SCBU 的新生儿中传播。
