Malaria Vaccine and Drug Development Center, Cali, Colombia.
Am J Trop Med Hyg. 2011 Feb;84(2 Suppl):51-7. doi: 10.4269/ajtmh.2011.09-0785.
Circumsporozoite (CS) protein is a malaria antigen involved in sporozoite invasion of hepatocytes, and thus considered to have good vaccine potential. We evaluated the polymorphism of the Plasmodium vivax CS gene in 24 parasite isolates collected from malaria-endemic areas of Colombia. We sequenced 27 alleles, most of which (25/27) corresponded to the VK247 genotype and the remainder to the VK210 type. All VK247 alleles presented a mutation (Gly → Asn) at position 28 in the N-terminal region, whereas the C-terminal presented three insertions: the ANKKAGDAG, which is common in all VK247 isolates; 12 alleles presented the insertion GAGGQAAGGNAANKKAGDAG; and 5 alleles presented the insertion GGNAGGNA. Both repeat regions were polymorphic in gene sequence and size. Sequences coding for B-, T-CD4(+), and T-CD8(+) cell epitopes were found to be conserved. This study confirms the high polymorphism of the repeat domain and the highly conserved nature of the flanking regions.
环子孢子蛋白（CS）是一种疟原虫抗原，参与子孢子侵入肝细胞，因此被认为具有良好的疫苗潜力。我们评估了从哥伦比亚疟疾流行地区采集的 24 株寄生虫分离株中，间日疟原虫 CS 基因的多态性。我们对 27 个等位基因进行了测序，其中大多数（25/27）与 VK247 基因型相对应，其余与 VK210 型相对应。所有 VK247 等位基因在 N 末端区域的第 28 位都存在一个突变（甘氨酸→天冬酰胺），而 C 末端则存在三个插入：ANKKKGDA，这在所有 VK247 分离株中都很常见；12 个等位基因具有插入 GAGGQAAGGNAANKKKGDA；5 个等位基因具有插入 GGNAGGNA。重复区在基因序列和大小上都具有多态性。发现编码 B、T-CD4(+)和 T-CD8(+)细胞表位的序列是保守的。这项研究证实了重复区的高度多态性和侧翼区的高度保守性。
