Grupo Salud y Comunidad, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia.
Acta Trop. 2011 Jul;119(1):23-9. doi: 10.1016/j.actatropica.2011.03.012. Epub 2011 Apr 7.
Genetic diversity of Plasmodium populations has been more extensively documented in Colombia for Plasmodium falciparum than for Plasmodium vivax. Recently, highly variable microsatellite markers have been described and used in population-level studies of genetic variation of P. vivax throughout the world. We applied this approach to understand the genetic structure of P. vivax populations and to identify recurrence-associated haplotypes. In this, three microsatellite markers of P. vivax were amplified and the combined size of the fragments was used to establish genotypes. Patients from an ongoing treatment efficacy trial who were kept either in endemic or non-endemic regions in the northwest of Colombia were included in the study. In total 58 paired clinical isolates, were amplified. A total of 54 haplotypes were observed among the two regions. Some haplotypes were exclusive to the endemic region where the highest degree of polymorphism was detected. In addition, we confirmed the different genotypes of recurrent-relapsing and primary infection isolates suggesting the activation of heterologous hypnozoite populations. We conclude that analysis of the three microsatellites is a valuable tool to establish the genetic characteristics of P. vivax populations in Colombia.
与恶性疟原虫（Plasmodium falciparum）相比，哥伦比亚对间日疟原虫（Plasmodium vivax）的种群遗传多样性有更广泛的记载。最近，高变异性微卫星标记已经被描述并用于世界各地间日疟原虫遗传变异的种群水平研究。我们应用这种方法来了解间日疟原虫种群的遗传结构，并确定与复发相关的单倍型。在本研究中，扩增了三个间日疟原虫微卫星标记，并使用片段的综合大小来建立基因型。本研究纳入了正在进行的疗效试验中的患者，这些患者分别来自哥伦比亚西北部的流行区和非流行区。共扩增了 58 对临床分离株。在这两个地区共观察到 54 种单倍型。一些单倍型是流行区所特有的，在那里检测到了最高程度的多态性。此外，我们还证实了复发-再燃和初次感染分离株的不同基因型，这表明异源休眠子群体被激活。我们的结论是，对这三个微卫星的分析是建立哥伦比亚间日疟原虫种群遗传特征的一种有价值的工具。
