Rickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Am J Trop Med Hyg. 2011 Feb;84(2):244-9. doi: 10.4269/ajtmh.2011.10-0355.
A recurrent focus of Rhipicephalus sanguineus infestation was investigated in a suburban area of southern California after reports of suspected Rocky Mountain spotted fever in two dogs on the same property. Abundant quantities of Rh. sanguineus were collected on the property and repeatedly from each dog, and Rickettsia massiliae DNA was detected by polymerase chain reaction (PCR). Whole blood and serum samples from four dogs were tested by using PCR and microimmunofluorescent assay for antibodies against spotted fever group rickettsiae. Serum samples from all four dogs contained antibodies reactive with R. massiliae, R. rhipicephali, R. rickettsii, and 364D Rickettsia but no rickettsial DNA was detected by PCR of blood samples. Serum cross-absorption and Western blot assays implicated R. massiliae as the most likely spotted fever group rickettsiae responsible for seropositivity. To our knowledge, this is the first detection of R. massiliae in ticks in California.
在加利福尼亚州南部的一个郊区，有两只要害地域斑疹热的狗出现在同一处，报告后，对硬蜱属（Rhipicephalus）血蜱的反复寄生现象进行了调查。在该物业上以及每只狗身上都采集到了大量的硬蜱属血蜱，并用聚合酶链反应（PCR）检测到了马氏泰勒虫（Rickettsia massiliae）的 DNA。对四只狗的全血和血清样本进行了检测，采用 PCR 和微免疫荧光分析检测针对斑点热群立克次体的抗体。四只狗的血清样本均与马氏泰勒虫、壁虱泰勒虫、立氏立克次体和 364D 立克次体反应，而血液样本的 PCR 检测未检测到立克次体 DNA。血清交叉吸收和 Western blot 分析表明马氏泰勒虫最有可能是导致血清阳性的斑点热群立克次体。据我们所知，这是加利福尼亚首次在蜱中检测到马氏泰勒虫。
