Universidade de Fortaleza, Brazil.
Am J Trop Med Hyg. 2011 Feb;84(2):302-5. doi: 10.4269/ajtmh.2011.10-0220.
A seroepidemiological investigation was conducted among the population of two municipalities in Northeastern Brazil. Immunoglobulin M (IgM) and IgG antibodies to Burkholderia pseudomallei were positive in 51.27% (161 in 317 samples) and 58.49% (186), respectively. IgM titers were higher in children than in adults. On the contrary, IgG increased progressively with age. We observed a significant association between agricultural occupation and raised IgM titers (P < 0.005) and IgG titers (P < 0.001), and between construction workers and raised IgG titers (P = 0.005). Antibody IgG avidities did not correlate with age. The highest titers of antibodies (1/800) showed the highest antibody avidity indexes (P < 0.01). Most of the serum samples recognized 45-kDa and 200-kDa bands by IgG1 and IgG2 subclasses. Our study showed a high seropositivity among individuals living in endemic regions of the state of Ceará, and highlights the need for further surveillance close to water courses such as dams and rivers in Northeastern Brazil.
本研究对巴西东北部两个城市的人群进行了血清流行病学调查。在 317 份样本中，161 份（51.27%）的布氏杆菌属假单胞菌免疫球蛋白 M（IgM）和 IgG 抗体以及 186 份（58.49%）的抗体呈阳性。IgM 滴度在儿童中高于成人。相反，IgG 随年龄逐渐增加。我们观察到农业职业与升高的 IgM 滴度（P < 0.005）和 IgG 滴度（P < 0.001）之间存在显著相关性，建筑工人与升高的 IgG 滴度（P = 0.005）之间存在显著相关性。抗体 IgG 的亲和性与年龄无关。抗体滴度最高（1/800）的抗体亲和指数最高（P < 0.01）。大多数血清样本通过 IgG1 和 IgG2 亚类识别 45-kDa 和 200-kDa 条带。本研究显示生活在塞阿拉州流行地区的个体存在高血清阳性率，强调需要在巴西东北部靠近水坝和河流等水道的地方进行进一步监测。
