Yui Sho, Ito Daisuke, Fujita Naoki, Nishimura Ryohei
Laboratory of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
J Vet Med Sci. 2011 Jan;73(1):133-7. doi: 10.1292/jvms.10-0344.
Olfactory ensheathing cells (OECs) have been reported to promote axonal regeneration when transplanted to rodent spinal cord injury models. OECs are available from the olfactory bulb (OB) and olfactory mucosa (OM). Although harvesting OECs from the OM is less traumatic, OECs originating from the OM are less proliferative than those from the OB (OB-OECs). One possible reason for this difference is coexisting fibroblasts. Here, we examined the effect of coculturing either fibroblasts from the OB (OB-Fibs) or fibroblasts from the OM (OM-Fibs) on the proliferation of OB-OECs. Proliferation of OB-OECs was significantly higher in 5:5 coculture with OB-Fibs and in 7:3 and 5:5 cocultures with OM-Fibs than without fibroblasts. These results indicated that coculture with both OB-Fibs and OM-Fibs promoted the proliferation of OB-OECs.
据报道，嗅鞘细胞（OECs）移植到啮齿动物脊髓损伤模型中时可促进轴突再生。OECs可从嗅球（OB）和嗅黏膜（OM）获取。虽然从OM获取OECs的创伤较小，但源自OM的OECs的增殖能力低于源自OB的OECs（OB - OECs）。造成这种差异的一个可能原因是共存的成纤维细胞。在此，我们研究了共培养来自OB的成纤维细胞（OB - Fibs）或来自OM的成纤维细胞（OM - Fibs）对OB - OECs增殖的影响。与不与成纤维细胞共培养相比，OB - OECs与OB - Fibs以5:5比例共培养以及与OM - Fibs以7:3和5:5比例共培养时，其增殖显著更高。这些结果表明，与OB - Fibs和OM - Fibs共培养均能促进OB - OECs的增殖。
