Evaluation of polyvalent anti-Pseudomonas aeruginosa vaccine in phagocytic reaction.

In order to obtain immune reaction for all known Pseudomonas aeruginosa immunotypes (7 according to Fisher), mucoid and nonmucoid variants, rabbits were immunized with polyvalent vaccine consisting of 3 mucoid (1, 3, 5) and 4 nonmucoid (2, 4, 6, 7) immunotypes. The serum of immunized animals induced an increase in normal rabbit granulocyte phagocytosis level as well as intracellular killing of all Pseudomonas aeruginosa immunotypes, both mucoid and nonmucoid variants. The serum was also observed to have high agglutinin titer (1:1024-1:8192) for all the bacteria. While the cross affinity of the mucoid antigens in polyvalent vaccine it was possible to reduce by 4 the number of the mucoid antigens used for immunization.