Department of Microbiology, Faculty of Biology, A. Mickiewicz University, Ul. Umultowska 89, 61-614, Poznań, Poland.
Antonie Van Leeuwenhoek. 2011 May;99(4):817-24. doi: 10.1007/s10482-011-9555-4. Epub 2011 Feb 4.
We investigated phylogenetic affiliation, occurrence of virulence genes and quinolone resistance in 109 integron-containing strains of Escherichia coli isolated from a wastewater treatment plant. Selection for integron-bearing strains caused a shift toward phylogroup D, which was most numerous, followed by A, B1 and B2. Phylogroups D and B2, both of which are reported to include virulent extraintestinal pathotypes, made up 50.5% of all isolates and were present in every stage of wastewater treatment, including final effluent. Diarrheagenic pathotypes made up 21% of the strains. The average virulence factor genes score was low (1.40) and the range was from 0 to 5. Quinolone and fluoroquinolone resistance was observed in 56.0% and 50.4% of the strains, respectively; however, it was not associated with virulence factor score. Although the average virulence factor score was low, 17.4% of strains had three and more virulence genes. They were isolated mostly from raw sewage, but 30% of them were cultured from final effluent. Release of multiresistant integron-bearing E. coli strains with virulence traits into the environment may create potential threat and be of public health concern.
我们调查了从一家污水处理厂分离的 109 株含整合子的大肠杆菌的系统发育相关性、毒力基因的发生情况和喹诺酮耐药性。对含整合子菌株的选择导致了向数量最多的 D 群 phylogroup 的转移，其次是 A、B1 和 B2。报道称，D 群和 B2 群都包括有致病性的肠外途径，占所有分离株的 50.5%，存在于污水处理的各个阶段，包括最终的流出物。腹泻性途径占菌株的 21%。平均毒力因子基因评分较低（1.40），范围为 0 至 5。分别有 56.0%和 50.4%的菌株对喹诺酮类和氟喹诺酮类产生耐药性；然而，这与毒力因子评分无关。尽管平均毒力因子评分较低，但 17.4%的菌株有三个或更多的毒力基因。它们主要从原始污水中分离出来，但其中 30%是从最终流出物中培养出来的。将具有毒力特征的多药耐药整合子携带大肠杆菌菌株释放到环境中可能会造成潜在威胁，并引起公共卫生关注。
