来自缅甸薯蓣的抗肺癌细胞细胞毒性皂苷。

Cytotoxic saponin against lung cancer cells from Dioscorea birmanica Prain & Burkill.

作者信息

Jaiaree Nuanjan, Itharat Arunporn, Kumapava Kamman

机构信息

Faculty of Medicine, Thammasat University, Pathumthani, Thailand.

出版信息

J Med Assoc Thai. 2010 Dec;93 Suppl 7:S192-7.

PMID:21294414

Abstract

OBJECTIVE

To investigate the cytotoxic activity against lung cancer cells of Dioscorea birmanica Prain & Burkill extract and its compounds.

MATERIAL AND METHOD

Cytotoxic activity was tested against two types of lung cancer cell line (A549 and CORL-23), one type of normal lung cell (MRC-5) by sulforhodamine B assay. Bioassay guide fractionation was used for isolating cytotoxic compounds. The structure elucidation of active ingredients was proven by spectrophotometry technique.

RESULTS

The results found that the ethanolic extract of Dioscorea birmanica Prain & Burkill (DBE) showed high cytotoxic activity against lung cancer cells; A 549 and COR-L23 (IC50 = 7.45 +/- 0.31, 8.71 +/- 0.29 microg/ml, respectively) but no cytotoxic activity against normal cancer cells MRC-5 (IC50 = 94.76 +/- 1.25 micro/ml). Six fractions from DBE were isolated by vaccuum liquid chromatography with ordering polarity of solvent and were coded as DB1-DB6, respectively. The fraction DB5 showed high cytotoxic against A 549 and COR-L23 (IC50 = 6.14 +/- 0.08 and 16.44 +/- 1.23 microg/ml, respectively) but less toxic to normal cell. Diosgenin-3-O-alpha-L-rhamnosyl (1-->2)-beta-D-glucopyranoside or Prosapogenin A of dioscin (DBS1) was isolated from DB5 fraction and had highest cytotoxic activity against those two types of lung cancer cells (IC50 = 1.81 +/- 0.03, 1.84 +/- 0.05 microg/ml), respectively) but less cytotoxic against normal lung cells MRC-5 (IC50 = 37.09 +/- 0.67 microg/ml).

CONCLUSION

The steroid saponin from Dioscorea birmanica showed cytotoxic activity against human lung cancer cells but less toxic against normal lung cells.

摘要

目的

研究薯蓣属植物缅甸薯蓣提取物及其化合物对肺癌细胞的细胞毒活性。

材料与方法

采用磺酰罗丹明B法检测缅甸薯蓣提取物对两种肺癌细胞系（A549和CORL-23）及一种正常肺细胞（MRC-5）的细胞毒活性。通过生物活性导向分离法分离细胞毒化合物。采用分光光度技术对活性成分进行结构鉴定。

结果

结果发现缅甸薯蓣乙醇提取物（DBE）对肺癌细胞具有较高的细胞毒活性；对A549和COR-L23的IC50分别为7.45±0.31、8.71±0.29μg/ml，但对正常癌细胞MRC-5无细胞毒活性（IC50 = 94.76±1.25μg/ml）。采用真空液相色谱法，按照溶剂极性顺序，从DBE中分离出6个组分，分别编码为DB1-DB6。组分DB5对A549和COR-L23具有较高的细胞毒活性（IC50分别为6.14±0.08和16.44±1.23μg/ml），但对正常细胞毒性较小。从DB5组分中分离出薯蓣皂苷元-3-O-α-L-鼠李糖基（1→2）-β-D-葡萄糖苷或薯蓣皂苷原A（DBS1），其对这两种肺癌细胞的细胞毒活性最高（IC50分别为1.81±0.03、1.84±0.05μg/ml），但对正常肺细胞MRC-5的细胞毒性较小（IC50 = 37.09±0.67μg/ml）。