Department of Chemistry, U.S. Department of Energy Ames Laboratory, Iowa State University, Ames, Iowa 50011-3111, USA.
ACS Nano. 2011 Feb 22;5(2):1366-75. doi: 10.1021/nn103077k. Epub 2011 Feb 4.
The interactions of mesoporous silica nanoparticles (MSNs) of different particle sizes and surface properties with human red blood cell (RBC) membranes were investigated by membrane filtration, flow cytometry, and various microscopic techniques. Small MCM-41-type MSNs (∼100 nm) were found to adsorb to the surface of RBCs without disturbing the membrane or morphology. In contrast, adsorption of large SBA-15-type MSNs (∼600 nm) to RBCs induced a strong local membrane deformation leading to spiculation of RBCs, internalization of the particles, and eventual hemolysis. In addition, the relationship between the degree of MSN surface functionalization and the degree of its interaction with RBC, as well as the effect of RBC-MSN interaction on cellular deformability, were investigated. The results presented here provide a better understanding of the mechanisms of RBC-MSN interaction and the hemolytic activity of MSNs and will assist in the rational design of hemocompatible MSNs for intravenous drug delivery and in vivo imaging.
通过膜过滤、流式细胞术和各种显微镜技术研究了不同粒径和表面性质的介孔硅纳米粒子（MSNs）与人类红细胞（RBC）膜的相互作用。发现小尺寸的 MCM-41 型 MSNs（约 100nm）在不破坏细胞膜或形态的情况下吸附在 RBC 表面。相比之下，大尺寸的 SBA-15 型 MSNs（约 600nm）吸附到 RBC 上会引起强烈的局部膜变形，导致 RBC 刺状化、颗粒内化，最终发生溶血。此外，还研究了 MSN 表面官能化程度与其与 RBC 相互作用程度之间的关系，以及 RBC-MSN 相互作用对细胞变形性的影响。本文的结果提供了对 RBC-MSN 相互作用机制和 MSNs 溶血活性的更好理解，并将有助于合理设计用于静脉内药物输送和体内成像的抗溶血 MSNs。
