Memelink J, van der Vlugt C I, Linthorst H J, Derks A F, Asjes C J, Bol J F
Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands.
J Gen Virol. 1990 Apr;71 ( Pt 4):917-24. doi: 10.1099/0022-1317-71-4-917.
cDNA clones complementary to the 3'-terminal regions of the genomic RNAs of the carlavirus lily symptomless virus and the potexvirus lily virus X (LVX) have been sequenced. The carlavirus RNA sequence contains five open reading frames (ORFs) coding for proteins of Mr 25,374, 11,631, 6960, 32,041 (coat protein) and 16,121, which are all very similar in size, amino acid sequence and relative position in the genome to proteins encoded by two different potato carlaviruses. The first four of these proteins also show considerable amino acid sequence similarity to proteins encoded by RNA of potexviruses, and the relative position of the ORFs on the carlavirus genome strongly resembles that in the potexvirus genomes. The LVX cDNA clone contains three ORFs encoding proteins of Mr 23,574, 11,767 and 21,569 (coat protein). A small ORF immediately 5' of the coat protein ORF, which has been found in other potexvirus genomes, is not present in the LVX genome. Thus, the data confirm the close taxonomic relationship between carlaviruses and potexviruses and reveal some differences in genome organization among the potexviruses.
已对香石竹潜隐病毒（一种香石竹病毒属病毒）和香石竹病毒X（LVX，一种马铃薯X病毒属病毒）基因组RNA 3'末端区域的互补cDNA克隆进行了测序。香石竹病毒属病毒的RNA序列包含5个开放阅读框（ORF），编码分子量分别为25374、11631、6960、32041（外壳蛋白）和16121的蛋白质，这些蛋白质在大小、氨基酸序列以及在基因组中的相对位置上，与两种不同马铃薯香石竹病毒属病毒编码的蛋白质都非常相似。这些蛋白质中的前四种与马铃薯X病毒属病毒RNA编码的蛋白质也显示出相当程度的氨基酸序列相似性，并且香石竹病毒属病毒基因组上开放阅读框的相对位置与马铃薯X病毒属病毒基因组中的情况极为相似。LVX cDNA克隆包含3个开放阅读框，编码分子量分别为23574、11767和21569（外壳蛋白）的蛋白质。在其他马铃薯X病毒属病毒基因组中发现的位于外壳蛋白开放阅读框紧邻5'端的一个小开放阅读框，在LVX基因组中不存在。因此，这些数据证实了香石竹病毒属病毒和马铃薯X病毒属病毒之间密切的分类学关系，并揭示了马铃薯X病毒属病毒之间基因组组织的一些差异。
