竹花叶病毒基因组RNA的核苷酸序列

Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus.

作者信息

Lin N S, Lin B Y, Lo N W, Hu C C, Chow T Y, Hsu Y H

机构信息

Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.

出版信息

J Gen Virol. 1994 Sep;75 ( Pt 9):2513-8. doi: 10.1099/0022-1317-75-9-2513.

DOI:10.1099/0022-1317-75-9-2513

PMID:8077956

Abstract

The complete nucleotide sequence of the genomic RNA of bamboo mosaic virus (BaMV) was determined by sequencing a set of overlapping cDNA clones and by direct sequencing of the viral RNA. The RNA genome of BaMV is 6366 nucleotides long [excluding 3'poly(A) tail] and contains six open reading frames (ORFs 1 to 6) coding for polypeptides with M(r) values of 155K, 28K, 13K, 6K, 25K and 14K, respectively. The genome organization and sizes of the encoded proteins are very similar to those of other potexviruses which have been sequenced except that ORF 6 lies completely within ORF 1. The first five putative proteins of the BaMV genome show identities ranging between 44 to 59%, 26 to 49%, 30 to 53%, 15 to 35% and 20 to 30%, respectively, to the corresponding ORFs of other members of the potexvirus group. However the putative product ORF 6 shows no significant similarity to those of other potexvirus ORF products.

摘要

通过对一组重叠的 cDNA 克隆进行测序以及对病毒 RNA 进行直接测序，确定了竹花叶病毒（BaMV）基因组 RNA 的完整核苷酸序列。BaMV 的 RNA 基因组长度为 6366 个核苷酸[不包括 3' 聚（A）尾]，包含六个开放阅读框（ORF1 至 ORF6），分别编码分子量为 155K、28K、13K、6K、25K 和 14K 的多肽。除了 ORF6 完全位于 ORF1 内之外，该病毒基因组的组织形式和所编码蛋白质的大小与已测序的其他马铃薯 X 病毒非常相似。BaMV 基因组的前五个推定蛋白与马铃薯 X 病毒组其他成员的相应 ORF 的同一性分别在 44%至 59%、26%至 49%、30%至 53%、15%至 35%和 20%至 30%之间。然而，推定产物 ORF6与其他马铃薯 X 病毒 ORF 产物没有明显的相似性。