Broad Institute, Cambridge, MA 02142, USA.
Cell. 2011 Feb 4;144(3):439-52. doi: 10.1016/j.cell.2010.12.032.
The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines. This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.
人类多能干细胞的发育潜力表明，它们可以产生与疾病相关的细胞类型，用于生物医学研究。然而，多能细胞系之间存在着大量的差异，这可能会影响它们的实用性和临床安全性。在将胚胎干细胞 (ES) 或诱导多能干细胞 (iPS) 细胞放心地用于转化研究之前，必须更好地了解这种细胞系特异性差异。为了实现这一目标，我们为 20 个先前衍生的人类 ES 系和 12 个人类 iPS 细胞系建立了全基因组 DNA 甲基化和基因表达的参考图谱，并测量了这些细胞系的体外分化倾向。这个资源使我们能够评估 ES 和 iPS 细胞的表观遗传学和转录相似性，并预测单个细胞系的分化效率。这些检测方法的组合提供了一个快速全面的多能细胞系特征描述的评分卡。
