Borjesson Dori L, Peroni John F
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, 95616, USA.
Clin Lab Med. 2011 Mar;31(1):109-23. doi: 10.1016/j.cll.2010.12.001.
This article focuses on the emerging field of equine regenerative medicine with an emphasis on the use of mesenchymal stem cells (MSCs) for orthopedic diseases. We detail laboratory procedures and protocols for tissue handling and MSC isolation, characterization, expansion, and cryopreservation from bone marrow, fat, and placental tissues. We provide an overview of current clinical uses for equine MSCs and how MSCs function to heal tissues. Current laboratory practices in equine regenerative medicine mirror those in the human field. However, the translational use of autologous and allogeneic MSCs for patient therapy far exceeds what is currently permitted in human medicine.
本文聚焦于新兴的马再生医学领域，重点关注间充质干细胞（MSCs）在骨科疾病中的应用。我们详细介绍了从骨髓、脂肪和胎盘组织中进行组织处理以及MSC分离、鉴定、扩增和冷冻保存的实验室程序和方案。我们概述了马MSCs目前的临床应用以及MSCs如何发挥作用来修复组织。马再生医学当前的实验室操作与人类领域的操作类似。然而，自体和异体MSCs在患者治疗中的转化应用远远超过了目前人类医学所允许的范围。
